Article,

Effect of Free Air CO\_2 Enrichment (FACE) and Nitrogen Level on Endopeptidase Activities in Rice Leaves During Grain Filling Stage

, , , , and .
Chinese Journal of Rice Science, (February 2008)

Abstract

A japonica rice variety Wuxiangjing 14 was grown under ambient(AMB) or elevated CO2(FACE,AMB + 200 μmol/mol) conitions and supplied with three levels of nitrogen: low level(LN,15 g/m2),normal level(NN,25 g /m2,similar to the level recommended to local farmers) and high level(HN,35 g/m2) to investigate the effect of elevated CO2 and N supply on endopeptidase(EP) activity and the specific activity in the flag and the second leaves from the top of rice.As compared with AMB,FACE significantly increased EP activity in the flag and the second leaves at 10 days after heading(DAH) and 20 DAH,as well as EP activity in the second leaves at heading.However,EP activity in the flag leaves at maturity was greatly reduced under FACE.FACE obviously increased the specific activity in the flag and the second leaves at 10,20 DAH and maturity,together with the specific activity in the second leaves at heading.In general,CO2 enrichment-induced changes in both EP activity and specific activity were larger at 10 and 20 DAH than at heading and maturity.EP activity at early filling stage decreased greatly with increasing N supply,while it showed adverse trends at late filling stages.The specific activity decreased with increasing N supply at all sampling stages,with CO2 enrichment-induced decreases being larger at early than at late filling stages.Compared with AMB,FACE significantly decreased the content of soluble protein in the flag and the second leaves at 10,20 DAH and maturity,and CO2 enrichment-induced changes in the content of soluble protein significantly decreased with increasing N supply.Soluble protein contents were correlated positively with EP activity in the flag and the second leaves at the maturity stage,while it showed adverse trends at the other sampling stages.This suggested that the CO2 enrichment-induced reduction in soluble protein content in leaves at the filling stage closely linked with corresponding changes in EP activity at elevated CO2.

Tags

Users

  • @dianella

Comments and Reviews