Artikel,

Single-Molecule STED Microscopy with Photostable Organic Fluorophores

, , , , , und .
Small, 6 (13): 1379--1384 (2010)
DOI: 10.1002/smll.201000203

Zusammenfassung

The combination of stimulated emission depletion (STED) microscopy with fluorophore stabilization through a reducing and oxidizing system (ROXS) enables the repetitive measurements necessary for 3D or dynamic STED imaging and resolution enhancement at the single-molecule level. A lateral resolution of <30 nm is obtained for raw image data recorded from single organic fluorophores immobilized in aqueous buffer

Tags

Nutzer

  • @reichert

Kommentare und Rezensionen