%0 Journal Article %1 Cherchi2010 %A Cherchi, Carla %A Gu, April Z. %D 2010 %J Environmental Science and Technology %K nanomaterials nanoparticles tio2 titanium\_dioxide toxicity %N 21 %P 8302-8307 %R 10.1021/es101658p %T Impact of Titanium Dioxide Nanomaterials on Nitrogen Fixation Rate and Intracellular Nitrogen Storage in Anabaena variabilis %U http://dx.doi.org/10.1021/es101658p %V 44 %X This study comprehensively investigated the impact of titanium dioxide nanomaterials (nTiO2) exposure on cell growth, nitrogen fixation activity, and nitrogen storage dynamics in the primary producer cyanobacteria Anabaena variabilis at various dose concentrations and exposure time lengths. The results indicated that both growth rate (EC50-96 h of 0.62 mgTiO2/L) and nitrogen fixation activity (EC50-96 h of 0.4 mgTiO2/L) were inhibited by nTiO2 exposure. The Hom's law (CnTm) was used as inactivation model to predict the concentration- and time-dependent inhibition of growth and nitrogen fixation activity. The kinetic parameters determined suggested that the time of exposure has a greater influence than the nTiO2 concentration in toxicity. We observed, for the first time, that nTiO2 induced a dose (concentration and time)-dependent increase in both the occurrence and intracellular levels of the nitrogen-rich cyanophycin grana proteins (CGPs). The results implied that CGPs may play an important role in the stress response mechanisms of nTiO2 exposure and can serve as a toxicity assessment endpoint indicator. This study demonstrated that nitrogen-fixing activity could be hampered by the release of nTiO2 in aquatic environments; therefore it potentially impacts important biogeochemical processes, such as carbon and nitrogen cycling.

@article{Cherchi2010, abstract = {This study comprehensively investigated the impact of titanium dioxide nanomaterials (nTiO2) exposure on cell growth, nitrogen fixation activity, and nitrogen storage dynamics in the primary producer cyanobacteria Anabaena variabilis at various dose concentrations and exposure time lengths. The results indicated that both growth rate (EC50-96 h of 0.62 mgTiO2/L) and nitrogen fixation activity (EC50-96 h of 0.4 mgTiO2/L) were inhibited by nTiO2 exposure. The Hom's law (CnTm) was used as inactivation model to predict the concentration- and time-dependent inhibition of growth and nitrogen fixation activity. The kinetic parameters determined suggested that the time of exposure has a greater influence than the nTiO2 concentration in toxicity. We observed, for the first time, that nTiO2 induced a dose (concentration and time)-dependent increase in both the occurrence and intracellular levels of the nitrogen-rich cyanophycin grana proteins (CGPs). The results implied that CGPs may play an important role in the stress response mechanisms of nTiO2 exposure and can serve as a toxicity assessment endpoint indicator. This study demonstrated that nitrogen-fixing activity could be hampered by the release of nTiO2 in aquatic environments; therefore it potentially impacts important biogeochemical processes, such as carbon and nitrogen cycling.}, added-at = {2011-10-01T00:23:31.000+0200}, author = {Cherchi, Carla and Gu, April Z.}, biburl = {https://www.bibsonomy.org/bibtex/2c2aadb14ef2e82f6cfbfb0fc040526ee/afcallender}, citeulike-article-id = {7866153}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/es101658p}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/es101658p}, doi = {10.1021/es101658p}, file = {Cherchi2010.pdf:indexed\\Cherchi2010.pdf:PDF}, groups = {public}, interhash = {59712668284c6f36eb65641085b11cd1}, intrahash = {c2aadb14ef2e82f6cfbfb0fc040526ee}, journal = {Environmental Science and Technology}, keywords = {nanomaterials nanoparticles tio2 titanium\_dioxide toxicity}, number = 21, pages = {8302-8307}, posted-at = {2010-09-21 00:28:54}, priority = {2}, timestamp = {2011-10-01T00:23:31.000+0200}, title = {Impact of Titanium Dioxide Nanomaterials on Nitrogen Fixation Rate and Intracellular Nitrogen Storage in Anabaena variabilis}, url = {http://dx.doi.org/10.1021/es101658p}, username = {afcallender}, volume = 44, year = 2010 }