Zusammenfassung
In this minireview we have attempted to provide some overall perspective
on the question of how various forms of diffusion in reduced dimensions,
or diffusion within a nonspecifically bound state, can speed biological
interactions beyond the limits normally set by three-dimensional
diffusion processes. To this end we began by discussing the rates
expected for small molecules engaged in classical elastic collisions.
We then proceeded to modify this picture by introducing first the
features of inelastic macromolecular collisions in solution, then
the effects of specific electrostatic fields set up around macromolecules
of known structure at low ionic strengths, and finally the special
rate enhancements available to DNA-protein interactions because
of the particular geometry of the DNA molecule and the multiple
conformations that can be assumed by the protein component. We hope
that this exposition will help to clarify the subject for others
and also will stimulate more focused examination of this type of
problem, both in DNA-protein interaction systems and in other biological
systems where such rate facilitation might apply.
- 2642903
- binding,
- dna,
- dna-binding
- factors,
- gov't,
- kinetics,
- models,
- non-u.s.
- operon,
- p.h.s.,
- protein
- proteins,
- repressor
- research
- support,
- theoretical,
- transcription
- u.s.
Nutzer