%0 Journal Article %1 Ookawa:2018:World-Neurosurg:29309973 %A Ookawa, S %A Wanibuchi, M %A Kataoka-Sasaki, Y %A Sasaki, M %A Oka, S %A Ohtaki, S %A Noshiro, S %A Komatsu, K %A Akiyama, Y %A Mikami, T %A Mikuni, N %A Kocsis, J D %A Honmou, O %D 2018 %J World Neurosurg %K cancer-research glioma marker paywall progression survival %P 783-783 %R 10.1016/j.wneu.2017.12.166 %T Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma %U https://www.ncbi.nlm.nih.gov/pubmed/29309973 %V 111 %X SERPINA1 plays an anti-inflammatory role in protecting tissues from proteolytic mechanisms. SERPINA1 is positive in gliomas by immunohistochemical analysis; however, the role of SERPINA1, including the relationship with prognosis, has been uncertain. In recent years, digital polymerase chain reaction (PCR) has provided ultra-sensitive assessment of messenger RNA expression from formalin-fixed paraffin-embedded (FFPE) tissues.In this study, we quantitatively determined the expression of SERPINA1 in high-grade gliomas (HGGs) using digital PCR, and we analyzed its relationship with prognosis.Twenty-nine FFPE surgical samples from patients with HGGs (7 of World Health Organization WHO grade III and 22 of WHO grade IV), and human glioblastoma cell lines, U87 and U118, were used for analysis. A qualitative assessment using immunostaining and quantitative assessment using digital PCR were performed to assess the expression of SERPINA1.The expression of SERPINA1 was demonstrated in glioma tissues and glioblastoma multiforme cell lines by immunostaining. Digital PCR analysis showed that SERPINA1 was expressed in 14.3% and 63.6% of the tissues from patients with grade III and grade IV HGG, respectively (P�= 0.035). The median overall survival of 38.8 months in the low SERPINA1 expression group was longer than that of 15.3 months in the high expression group (P�= 0.030).The frequency and the amount of SERPINA1 expression were higher in grade IV than in grade III HGGs. The high expression of SERPINA1 indicates a poor prognosis of HGGs.

@article{Ookawa:2018:World-Neurosurg:29309973, abstract = {SERPINA1 plays an anti-inflammatory role in protecting tissues from proteolytic mechanisms. SERPINA1 is positive in gliomas by immunohistochemical analysis; however, the role of SERPINA1, including the relationship with prognosis, has been uncertain. In recent years, digital polymerase chain reaction (PCR) has provided ultra-sensitive assessment of messenger RNA expression from formalin-fixed paraffin-embedded (FFPE) tissues.In this study, we quantitatively determined the expression of SERPINA1 in high-grade gliomas (HGGs) using digital PCR, and we analyzed its relationship with prognosis.Twenty-nine FFPE surgical samples from patients with HGGs (7 of World Health Organization [WHO] grade III and 22 of WHO grade IV), and human glioblastoma cell lines, U87 and U118, were used for analysis. A qualitative assessment using immunostaining and quantitative assessment using digital PCR were performed to assess the expression of SERPINA1.The expression of SERPINA1 was demonstrated in glioma tissues and glioblastoma multiforme cell lines by immunostaining. Digital PCR analysis showed that SERPINA1 was expressed in 14.3% and 63.6% of the tissues from patients with grade III and grade IV HGG, respectively (P�= 0.035). The median overall survival of 38.8 months in the low SERPINA1 expression group was longer than that of 15.3 months in the high expression group (P�= 0.030).The frequency and the amount of SERPINA1 expression were higher in grade IV than in grade III HGGs. The high expression of SERPINA1 indicates a poor prognosis of HGGs.}, added-at = {2018-05-18T23:54:38.000+0200}, author = {Ookawa, S and Wanibuchi, M and Kataoka-Sasaki, Y and Sasaki, M and Oka, S and Ohtaki, S and Noshiro, S and Komatsu, K and Akiyama, Y and Mikami, T and Mikuni, N and Kocsis, J D and Honmou, O}, biburl = {https://www.bibsonomy.org/bibtex/25ca80ce8247cf3203f66f980eaefdc5f/marcsaric}, description = {Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma. - PubMed - NCBI}, doi = {10.1016/j.wneu.2017.12.166}, interhash = {6957e81ee28d13501f85fe6aaf96f9ce}, intrahash = {5ca80ce8247cf3203f66f980eaefdc5f}, journal = {World Neurosurg}, keywords = {cancer-research glioma marker paywall progression survival}, month = mar, pages = {783-783}, pmid = {29309973}, timestamp = {2018-05-18T23:54:38.000+0200}, title = {Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma}, url = {https://www.ncbi.nlm.nih.gov/pubmed/29309973}, volume = 111, year = 2018 }