%0 Journal Article %1 Basile1993 %A Basile, Franco %A Cardamone, Anita %A Grinstead, Keith D. %A Miller, Karen J. %A Lytle, Fred E. %A Caprara, Andrea %A Clark, Chris D. %A Heritier, Jean-Marc %D 1993 %J Appl. Spectrosc. %K fluorescence hardware lifetime %N 2 %P 207-210 %R 10.1366/0003702934048325 %T Simple Time-Resolved Fluorometer Based on a Nanosecond Digital Oscilloscope and a Diode-Pumped, Solid-State Laser %V 47 %X A simple time-resolved fluorometer is constructed with an all-solid-state-based, frequency-tripled, Q-switched, diode-pumped Nd:YLF laser as the excitation source. Signal processing is accomplished with a digital oscilloscope. Simplicity of operation and applicability to trace analysis and in time-resolved spectroscopy are demonstrated with this new instrument. The laser produces 2.5-ns pulses at 349 nm and is capable of kilohertz repetition rates. For every shot of the laser, the oscilloscope collects an entire fluorescence decay at a 1-ns digitizer resolution and can average these data at the maximum laser repetition rate. When one is operating at 1 kHz and signal averaging for one second, detection limits (S/N = 3) in the 10-100 pM region are obtained. Excited-state decays are collected for several enzymatic probes and quinine sulfate, providing lifetimes consistent with those obtained by established instruments.

@article{Basile1993, abstract = {A simple time-resolved fluorometer is constructed with an all-solid-state-based, frequency-tripled, Q-switched, diode-pumped Nd:YLF laser as the excitation source. Signal processing is accomplished with a digital oscilloscope. Simplicity of operation and applicability to trace analysis and in time-resolved spectroscopy are demonstrated with this new instrument. The laser produces 2.5-ns pulses at 349 nm and is capable of kilohertz repetition rates. For every shot of the laser, the oscilloscope collects an entire fluorescence decay at a 1-ns digitizer resolution and can average these data at the maximum laser repetition rate. When one is operating at 1 kHz and signal averaging for one second, detection limits (S/N = 3) in the 10-100 pM region are obtained. Excited-state decays are collected for several enzymatic probes and quinine sulfate, providing lifetimes consistent with those obtained by established instruments.}, added-at = {2010-03-09T21:58:22.000+0100}, author = {Basile, Franco and Cardamone, Anita and Grinstead, Keith D. and Miller, Karen J. and Lytle, Fred E. and Caprara, Andrea and Clark, Chris D. and Heritier, Jean-Marc}, biburl = {https://www.bibsonomy.org/bibtex/22168330a2c08415860b4e4b5be4468da/afcallender}, doi = {10.1366/0003702934048325}, file = {Basile1993.pdf:indexed\\Basile1993.pdf:PDF}, interhash = {7a5b3f027d24b2d141c6a194a8185dc8}, intrahash = {2168330a2c08415860b4e4b5be4468da}, journal = {Appl. Spectrosc.}, keywords = {fluorescence hardware lifetime}, month = {February}, number = 2, pages = {207-210}, timestamp = {2010-03-09T22:20:10.000+0100}, title = {Simple Time-Resolved Fluorometer Based on a Nanosecond Digital Oscilloscope and a Diode-Pumped, Solid-State Laser}, volume = 47, year = 1993 }