%0 Journal Article %1 noauthororeditor2018establishing %A Pérez-González, Mariana Zuleima %A Nieto-Trujillo, Aurelio %A García-Martínez, Ignacio %A Estrada-Zúñiga, María Elena %A Bernabé-Antonio, Antonio %A Jimenez-Arellanes, María Adelina %A Cruz-Sosa, Francisco %D 2018 %J Advances in Biochemistry and Biotechnology %K Chaya In Medicinal Plant culture growth plants regulators vitro %N 02 %P 1-5 %T "Establishing a Callus Culture of Cnidoscolus chayamansa McVaugh: A Species with Ethnopharmacological Value" %U https://gavinpublishers.com/journals/artical_in_press/advances-in-biochemistry-and-biotechnology-ISSN-2574-7258 %V 2018 %X Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts or specific compounds of interest. Different concentrations and combinations of 6-Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Murashige and Skoog (MS) culture medium were evaluated for their ability to support callus induction from immature leaf explants. Highest callus yield (100%) was achieved when leaf explants were incubated in MS medium supplemented with BAP (5.0 mg L-1) and 2,4-D (2.5 or 5.0 mg mL-1). A reduced rate of callus induction (75%) was observed when leaf explants were exposed to lower concentrations (0.5 mg L-1) of BAP or 2,4-D. The calluses obtained in this work can be used as a biotechnological alternative for in vitro propagation of C. chayamansa and for obtaining extracts or compounds with a possible pharmacological application.

@article{noauthororeditor2018establishing, abstract = {Cnidoscolus chayamansa is a plant widely used as food with potential medicinal applications. Plant tissue culture enables the production of secondary metabolites from plants in a sustainable manner. The aim of this work was to establish a callus culture of C. chayamansa using leaf explants, which, in the future, can be used to obtain whole extracts or specific compounds of interest. Different concentrations and combinations of 6-Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic Acid (2,4-D) in Murashige and Skoog (MS) culture medium were evaluated for their ability to support callus induction from immature leaf explants. Highest callus yield (100%) was achieved when leaf explants were incubated in MS medium supplemented with BAP (5.0 mg L-1) and 2,4-D (2.5 or 5.0 mg mL-1). A reduced rate of callus induction (75%) was observed when leaf explants were exposed to lower concentrations (0.5 mg L-1) of BAP or 2,4-D. The calluses obtained in this work can be used as a biotechnological alternative for in vitro propagation of C. chayamansa and for obtaining extracts or compounds with a possible pharmacological application. }, added-at = {2019-05-02T09:21:04.000+0200}, author = {Pérez-González, Mariana Zuleima and Nieto-Trujillo, Aurelio and García-Martínez, Ignacio and Estrada-Zúñiga, María Elena and Bernabé-Antonio, Antonio and Jimenez-Arellanes, María Adelina and Cruz-Sosa, Francisco}, biburl = {https://www.bibsonomy.org/bibtex/2908b4447cc92394cd7432afb6438f280/alyssacarter}, interhash = {7beb45640a48e0f02f6a8c3ed8ebae7c}, intrahash = {908b4447cc92394cd7432afb6438f280}, issn = {ISSN: 2574-7258}, journal = {Advances in Biochemistry and Biotechnology }, keywords = {Chaya In Medicinal Plant culture growth plants regulators vitro}, language = {English}, month = {April }, number = 02, pages = {1-5}, timestamp = {2019-05-02T09:21:04.000+0200}, title = {"Establishing a Callus Culture of Cnidoscolus chayamansa McVaugh: A Species with Ethnopharmacological Value"}, url = {https://gavinpublishers.com/journals/artical_in_press/advances-in-biochemistry-and-biotechnology-ISSN-2574-7258}, volume = 2018, year = 2018 }