Article,
Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection
Analytica Chimica Acta, 608 (2): 204--210 (February 2008)
DOI: 10.1016/j.aca.2007.12.026
Abstract
A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2 M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 mug mL-1 for imipenem and 1.5 and 20 mug mL-1 for rifampicin. The detection limits were 0.07 mug mL-1 and 0.47 mug mL-1 for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.
