%0 Journal Article %1 castanheira_molecular_2004 %A Castanheira, Mariana %A Toleman, Mark A %A Jones, Ronald N %A Schmidt, Franz J %A Walsh, Timothy R %D 2004 %J Antimicrobial Agents and Chemotherapy %K Acid Agar Amino Bacterial, Carbapenems, Chain Data, Drug Electrophoresis, Focusing, Gel, Genes, Integrons, Isoelectric Kinetics, Microbial Molecular Phenotype, Polymerase Primers, Pseudomonas Reaction Resistance, Reverse Sensitivity Sequence Sequence, Tests, Transcriptase aeruginosa, {DNA,} {DNA} {Pulsed-Field,} {beta-Lactamases,} %N 12 %P 4654--4561 %R 48/12/4654 %T Molecular characterization of a beta-lactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase %U http://www.ncbi.nlm.nih.gov/pubmed/15561840 %V 48 %X As part of the SENTRY Antimicrobial Surveillance Program in 2002, five multidrug-resistant Pseudomonas aeruginosa clinical isolates were detected with metallo-beta-lactamase (MbetaL) activity. The isolates were recovered from different patients in a medical center located in Dusseldorf, Germany. The resistant determinant was isolated amplifying the region between the integrase and the aacA4 gene cassette. Sequencing revealed a novel MbetaL gene, designated bla(GIM-1). Additional analysis showed that GIM-1, comprising 250 amino acids and with a pI value of 5.4, differs in its primary sequence from that described for IMP, VIM, and SPM-1 enzymes by 39 to 43\%, 28 to 31\%, and 28\%, respectively. The enzyme possesses unique amino acids within the major consensus sequence (HXHXD) of the MbetaL family. Kinetics analysis revealed that GIM-1 has no clear preference for any substrate and did not hydrolyze azlocillin, aztreonam, and the serine-beta-lactamase inhibitors. bla(GIM-1) was found on a 22-kb nontransferable plasmid. The new MbetaL gene was embedded in the first position of a 6-kb class 1 integron, In77, with distinct features, including an aacA4 cassette downstream of the MbetaL gene that appeared to be truncated with bla(GIM-1). The aacA4 was followed by an aadA1 gene cassette that was interrupted by a copy of the IS1394. This integron also carried an oxacillinase gene, bla(OXA-2), before the 3'-CS region. GIM-1 appears to be a unique MbetaL, which is located in a distinct integron structure, and represents the fourth subclass of mobile MbetaL enzymes to be characterized.

@article{castanheira_molecular_2004, abstract = {As part of the {SENTRY} Antimicrobial Surveillance Program in 2002, five multidrug-resistant Pseudomonas aeruginosa clinical isolates were detected with metallo-beta-lactamase {(MbetaL)} activity. The isolates were recovered from different patients in a medical center located in Dusseldorf, Germany. The resistant determinant was isolated amplifying the region between the integrase and the {aacA4} gene cassette. Sequencing revealed a novel {MbetaL} gene, designated {bla(GIM-1).} Additional analysis showed that {GIM-1,} comprising 250 amino acids and with a {pI} value of 5.4, differs in its primary sequence from that described for {IMP,} {VIM,} and {SPM-1} enzymes by 39 to 43\%, 28 to 31\%, and 28\%, respectively. The enzyme possesses unique amino acids within the major consensus sequence {(HXHXD)} of the {MbetaL} family. Kinetics analysis revealed that {GIM-1} has no clear preference for any substrate and did not hydrolyze azlocillin, aztreonam, and the serine-beta-lactamase inhibitors. {bla(GIM-1)} was found on a 22-kb nontransferable plasmid. The new {MbetaL} gene was embedded in the first position of a 6-kb class 1 integron, In77, with distinct features, including an {aacA4} cassette downstream of the {MbetaL} gene that appeared to be truncated with {bla(GIM-1).} The {aacA4} was followed by an {aadA1} gene cassette that was interrupted by a copy of the {IS1394.} This integron also carried an oxacillinase gene, {bla(OXA-2),} before the {3'-CS} region. {GIM-1} appears to be a unique {MbetaL,} which is located in a distinct integron structure, and represents the fourth subclass of mobile {MbetaL} enzymes to be characterized.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Castanheira, Mariana and Toleman, Mark A and Jones, Ronald N and Schmidt, Franz J and Walsh, Timothy R}, biburl = {https://www.bibsonomy.org/bibtex/2e8792b2a628b6cf07f46c4e19882d8f3/jelias}, doi = {48/12/4654}, interhash = {8170d1e1d2537c988d9aff0cb9c9aafc}, intrahash = {e8792b2a628b6cf07f46c4e19882d8f3}, issn = {0066-4804}, journal = {Antimicrobial Agents and Chemotherapy}, keywords = {Acid Agar Amino Bacterial, Carbapenems, Chain Data, Drug Electrophoresis, Focusing, Gel, Genes, Integrons, Isoelectric Kinetics, Microbial Molecular Phenotype, Polymerase Primers, Pseudomonas Reaction Resistance, Reverse Sensitivity Sequence Sequence, Tests, Transcriptase aeruginosa, {DNA,} {DNA} {Pulsed-Field,} {beta-Lactamases,}}, month = dec, note = {{PMID:} 15561840}, number = 12, pages = {4654--4561}, timestamp = {2011-03-11T10:05:55.000+0100}, title = {Molecular characterization of a beta-lactamase gene, {blaGIM-1,} encoding a new subclass of metallo-beta-lactamase}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15561840}, volume = 48, year = 2004 }