Imaging microdomain Ca$^2+$ in muscle cells.
S. Wang, C. Wei, G. Zhao, D. Brochet, J. Shen, L. Song, W. Wang, D. Yang, and H. Cheng.
Circ. Res. 94 (8): 1011--1022 (April 2004)

Ca$^2+$ ions passing through a single or a cluster of Ca$^2+$-permeable channels create microscopic, short-lived Ca$^2+$ gradients that constitute the building blocks of cellular Ca$^2+$ signaling. Over the last decade, imaging microdomain Ca$^2+$ in muscle cells has unveiled the exquisite spatial and temporal architecture of intracellular Ca$^2+$ dynamics and has reshaped our understanding of Ca$^2+$ signaling mechanisms. Major advances include the visualization of "Ca$^2+$ sparks" as the elementary events of Ca$^2+$ release from the sarcoplasmic reticulum (SR), "Ca$^2+$ sparklets" produced by openings of single Ca$^2+$-permeable channels, miniature Ca$^2+$ transients in single mitochondria ("marks"), and SR luminal Ca$^2+$ depletion transients ("scraps"). As a model system, a cardiac myocyte contains a 3-dimensional grid of 104 spark ignition sites, stochastic activation of which summates into global Ca$^2+$ transients. Tracking intermolecular coupling between single L-type Ca$^2+$ channels and Ca$^2+$ sparks has provided direct evidence validating the local control theory of Ca$^2+$-induced Ca$^2+$ release in the heart. In vascular smooth muscle myocytes, Ca$^2+$ can paradoxically signal both vessel constriction (by global Ca$^2+$ transients) and relaxation (by subsurface Ca$^2+$ sparks). These findings shed new light on the origin of Ca$^2+$ signaling efficiency, specificity, and versatility. In addition, microdomain Ca$^2+$ imaging offers a novel modality that complements electrophysiological approaches in characterizing Ca$^2+$ channels in intact cells.

URL: http://dx.doi.org/10.1161/01.RES.0000125883.68447.A1
DOI: 10.1161/01.RES.0000125883.68447.A1
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@article{Wang_2004_1011,
  abstract = {{C}a$^{2+}$ ions passing through a single or a cluster of {C}a$^{2+}$-permeable
	channels create microscopic, short-lived {C}a$^{2+}$ gradients that
	constitute the building blocks of cellular {C}a$^{2+}$ signaling.
	Over the last decade, imaging microdomain {C}a$^{2+}$ in muscle cells
	has unveiled the exquisite spatial and temporal architecture of intracellular
	{C}a$^{2+}$ dynamics and has reshaped our understanding of {C}a$^{2+}$
	signaling mechanisms. Major advances include the visualization of
	"{C}a$^{2+}$ sparks" as the elementary events of {C}a$^{2+}$ release
	from the sarcoplasmic reticulum (SR), "{C}a$^{2+}$ sparklets" produced
	by openings of single {C}a$^{2+}$-permeable channels, miniature {C}a$^{2+}$
	transients in single mitochondria ("marks"), and SR luminal {C}a$^{2+}$
	depletion transients ("scraps"). As a model system, a cardiac myocyte
	contains a 3-dimensional grid of 104 spark ignition sites, stochastic
	activation of which summates into global {C}a$^{2+}$ transients.
	Tracking intermolecular coupling between single L-type {C}a$^{2+}$
	channels and {C}a$^{2+}$ sparks has provided direct evidence validating
	the local control theory of {C}a$^{2+}$-induced {C}a$^{2+}$ release
	in the heart. In vascular smooth muscle myocytes, {C}a$^{2+}$ can
	paradoxically signal both vessel constriction (by global {C}a$^{2+}$
	transients) and relaxation (by subsurface {C}a$^{2+}$ sparks). These
	findings shed new light on the origin of {C}a$^{2+}$ signaling efficiency,
	specificity, and versatility. In addition, microdomain {C}a$^{2+}$
	imaging offers a novel modality that complements electrophysiological
	approaches in characterizing {C}a$^{2+}$ channels in intact cells.},
  added-at = {2009-06-03T11:20:58.000+0200},
  author = {Wang, Shi-Qiang and Wei, Chaoliang and Zhao, Guiling and Brochet, Didier X P and Shen, Jianxin and Song, Long-Sheng and Wang, Wang and Yang, Dongmei and Cheng, Heping},
  biburl = {https://www.bibsonomy.org/bibtex/2c56f361c1da6abe144132ec34669481e/hake},
  description = {The whole bibliography file I use.},
  doi = {10.1161/01.RES.0000125883.68447.A1},
  file = {Wang_2004_1011.pdf:Wang_2004_1011.pdf:PDF},
  interhash = {827bf5c0bf75fa77f343ece976cf9156},
  intrahash = {c56f361c1da6abe144132ec34669481e},
  journal = {Circ. Res.},
  key = 162,
  keywords = {15117829 Abstract, Acid, Agents, Animals, Araceae, CHO Calcium Calcium, Carcinoma, Cardiac, Cell Cells, Channel Channel, Channels, Chelating Chinese Confocal, Drugs, Egtazic English Expression Gating, Gene Gov't, Hamsters, Heart, Hepatocellular, Herbal, Humans, Ion L-Type, Line, Liver Medicinal, Microscopy, Mitochondria, Muscle, Myocytes, Neoplasm, Neoplasms, Neoplastic, Non-U.S. P.H.S., Patch-Clamp Plants, Profiling, RNA, Rabbits, Rats, Receptor Regulation, Release Research Reticulum, Rhizome, Ryanodine Sarcoplasmic Signaling, Smooth Smooth, Support, Techniques, Transport, Tumor, U.S. Vascular,},
  month = Apr,
  number = 8,
  pages = {1011--1022},
  pii = {94/8/1011},
  pmid = {15117829},
  timestamp = {2009-06-03T11:21:36.000+0200},
  title = {Imaging microdomain {C}a$^{2+}$ in muscle cells.},
  url = {http://dx.doi.org/10.1161/01.RES.0000125883.68447.A1},
  volume = 94,
  year = 2004
}

%0 Journal Article
%1 Wang_2004_1011
%A Wang, Shi-Qiang
%A Wei, Chaoliang
%A Zhao, Guiling
%A Brochet, Didier X P
%A Shen, Jianxin
%A Song, Long-Sheng
%A Wang, Wang
%A Yang, Dongmei
%A Cheng, Heping
%D 2004
%J Circ. Res.
%K 15117829 Abstract, Acid, Agents, Animals, Araceae, CHO Calcium Calcium, Carcinoma, Cardiac, Cell Cells, Channel Channel, Channels, Chelating Chinese Confocal, Drugs, Egtazic English Expression Gating, Gene Gov't, Hamsters, Heart, Hepatocellular, Herbal, Humans, Ion L-Type, Line, Liver Medicinal, Microscopy, Mitochondria, Muscle, Myocytes, Neoplasm, Neoplasms, Neoplastic, Non-U.S. P.H.S., Patch-Clamp Plants, Profiling, RNA, Rabbits, Rats, Receptor Regulation, Release Research Reticulum, Rhizome, Ryanodine Sarcoplasmic Signaling, Smooth Smooth, Support, Techniques, Transport, Tumor, U.S. Vascular,
%N 8
%P 1011--1022
%R 10.1161/01.RES.0000125883.68447.A1
%T Imaging microdomain Ca$^2+$ in muscle cells.
%U http://dx.doi.org/10.1161/01.RES.0000125883.68447.A1
%V 94
%X Ca$^2+$ ions passing through a single or a cluster of Ca$^2+$-permeable
	channels create microscopic, short-lived Ca$^2+$ gradients that
	constitute the building blocks of cellular Ca$^2+$ signaling.
	Over the last decade, imaging microdomain Ca$^2+$ in muscle cells
	has unveiled the exquisite spatial and temporal architecture of intracellular
	Ca$^2+$ dynamics and has reshaped our understanding of Ca$^2+$
	signaling mechanisms. Major advances include the visualization of
	"Ca$^2+$ sparks" as the elementary events of Ca$^2+$ release
	from the sarcoplasmic reticulum (SR), "Ca$^2+$ sparklets" produced
	by openings of single Ca$^2+$-permeable channels, miniature Ca$^2+$
	transients in single mitochondria ("marks"), and SR luminal Ca$^2+$
	depletion transients ("scraps"). As a model system, a cardiac myocyte
	contains a 3-dimensional grid of 104 spark ignition sites, stochastic
	activation of which summates into global Ca$^2+$ transients.
	Tracking intermolecular coupling between single L-type Ca$^2+$
	channels and Ca$^2+$ sparks has provided direct evidence validating
	the local control theory of Ca$^2+$-induced Ca$^2+$ release
	in the heart. In vascular smooth muscle myocytes, Ca$^2+$ can
	paradoxically signal both vessel constriction (by global Ca$^2+$
	transients) and relaxation (by subsurface Ca$^2+$ sparks). These
	findings shed new light on the origin of Ca$^2+$ signaling efficiency,
	specificity, and versatility. In addition, microdomain Ca$^2+$
	imaging offers a novel modality that complements electrophysiological
	approaches in characterizing Ca$^2+$ channels in intact cells.

BibSonomy

Imaging microdomain Ca$^2+$ in muscle cells.
S. Wang, C. Wei, G. Zhao, D. Brochet, J. Shen, L. Song, W. Wang, D. Yang, and H. Cheng.
Circ. Res. 94 (8): 1011--1022 (April 2004)

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BibSonomy

Imaging microdomain Ca$^2+$ in muscle cells.S. Wang, C. Wei, G. Zhao, D. Brochet, J. Shen, L. Song, W. Wang, D. Yang, and H. Cheng. Circ. Res. 94 (8): 1011--1022 (April 2004)

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Cite this publication

Imaging microdomain Ca$^2+$ in muscle cells.
S. Wang, C. Wei, G. Zhao, D. Brochet, J. Shen, L. Song, W. Wang, D. Yang, and H. Cheng.
Circ. Res. 94 (8): 1011--1022 (April 2004)