%0 Journal Article %1 bae_determination_2008 %A Bae, Soo Kyung %A Seo, Kyung Ah %A Jung, Eun Ji %A Kim, Ho-Sook %A Yeo, Chang-Woo %A Shon, Ji-Hong %A Park, Kyung-Mi %A Liu, Kwang-Hyeon %A Shin, Jae-Gook %D 2008 %J Biomedical Chromatography: BMC %K Acid, Aspirin, Humans, Korea, Mass Reference Reproducibility Results, Salicylic Sensitivity Specificity, Spectrometry Standards, Tandem Values, and of %N 6 %P 590--595 %R 10.1002/bmc.973 %T Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study of Astrix in Korean healthy volunteers %U http://www.ncbi.nlm.nih.gov/pubmed/18254152 %V 22 %X The first liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for determination of acetylsalicylic acid (aspirin, ASA) and one of its major metabolites, salicylic acid (SA), in human plasma using simvastatin as an internal standard has been developed and validated. For ASA analysis, a plasma sample containing potassium fluoride was extracted using a mixture of ethyl acetate and diethyl ether in the presence of 0.5\% formic acid. SA, a major metabolite of ASA, was extracted from plasma using protein precipitation with acetonitrile. The compounds were separated on a reversed-phase column with an isocratic mobile phase consisting of acetonitrile and water containing 0.1\% formic acid (8:2, v/v). The ion transitions recorded in multiple reaction monitoring mode were m/z 179 --\textgreater 137, 137 --\textgreater 93 and 435 --\textgreater 319 for ASA, SA and IS, respectively. The coefficient of variation of the assay precision was less than 9.3\%, and the accuracy exceeded 86.5\%. The lower limits of quantification for ASA and SA were 5 and 50 ng/mL, respectively. The developed assay method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after single oral administration of Astrix (entero-coated pellet, 100 mg of aspirin) to 10 Korean healthy male volunteers.

@article{bae_determination_2008, abstract = {The first liquid chromatography-tandem mass spectrometry {(LC/MS/MS)} method for determination of acetylsalicylic acid (aspirin, {ASA)} and one of its major metabolites, salicylic acid {(SA),} in human plasma using simvastatin as an internal standard has been developed and validated. For {ASA} analysis, a plasma sample containing potassium fluoride was extracted using a mixture of ethyl acetate and diethyl ether in the presence of 0.5\% formic acid. {SA,} a major metabolite of {ASA,} was extracted from plasma using protein precipitation with acetonitrile. The compounds were separated on a reversed-phase column with an isocratic mobile phase consisting of acetonitrile and water containing 0.1\% formic acid (8:2, v/v). The ion transitions recorded in multiple reaction monitoring mode were m/z 179 --{\textgreater} 137, 137 --{\textgreater} 93 and 435 --{\textgreater} 319 for {ASA,} {SA} and {IS,} respectively. The coefficient of variation of the assay precision was less than 9.3\%, and the accuracy exceeded 86.5\%. The lower limits of quantification for {ASA} and {SA} were 5 and 50 {ng/mL,} respectively. The developed assay method was successfully applied for the evaluation of pharmacokinetics of {ASA} and {SA} after single oral administration of Astrix (entero-coated pellet, 100 mg of aspirin) to 10 Korean healthy male volunteers.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Bae, Soo Kyung and Seo, Kyung Ah and Jung, Eun Ji and Kim, {Ho-Sook} and Yeo, {Chang-Woo} and Shon, {Ji-Hong} and Park, {Kyung-Mi} and Liu, {Kwang-Hyeon} and Shin, {Jae-Gook}}, biburl = {https://www.bibsonomy.org/bibtex/28ca6ad128023287d3941a52ec00c6f2a/jelias}, doi = {10.1002/bmc.973}, interhash = {8df51ea0f4ac421dbf0765a2376b49cb}, intrahash = {8ca6ad128023287d3941a52ec00c6f2a}, issn = {0269-3879}, journal = {Biomedical Chromatography: {BMC}}, keywords = {Acid, Aspirin, Humans, Korea, Mass Reference Reproducibility Results, Salicylic Sensitivity Specificity, Spectrometry Standards, Tandem Values, and of}, month = jun, note = {{PMID:} 18254152}, number = 6, pages = {590--595}, shorttitle = {Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography-tandem mass spectrometry}, timestamp = {2011-03-11T10:06:35.000+0100}, title = {Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study of Astrix in Korean healthy volunteers}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18254152}, volume = 22, year = 2008 }