%0 Journal Article %1 citeulike:700744 %A Dempsey, E. %A O'Sullivan, C. %A Smyth, M. R. %A Egan, D. %A O'Kennedy, R. %A Wang, J. %C School of Chemical Sciences, Dublin City University, Ireland. %D 1993 %J Analyst %K amperometry antibody electrochemistry immunoelectrode %N 4 %P 411--413 %T Development of an antibody-based amperometric biosensor to study the reaction of 7-hydroxycoumarin with its specific antibody. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8494174 %V 118 %X An amperometric biosensor, based on an anti-7-hydroxycoumarin (7-OH-coumarin) antibody immobilized at the surface of a glassy carbon electrode, and contained behind a cellulose dialysis membrane, is described. The electrochemical behaviour of this metabolite at the bare glassy carbon electrode was found to be well defined by using differential-pulse voltammetry, and a 90% decrease in peak height was observed on binding of the antibody to the antigen, which occurs at the electroactive site of 7-OH-coumarin. The kinetics of the antibody-antigen reaction was investigated and a first-order reaction was observed with k = 0.0329 mA min-1. This system provides a novel method for studying antibody specificity and the kinetics of such antibody-antigen interactions.

@article{citeulike:700744, abstract = {An amperometric biosensor, based on an anti-7-hydroxycoumarin (7-OH-coumarin) antibody immobilized at the surface of a glassy carbon electrode, and contained behind a cellulose dialysis membrane, is described. The electrochemical behaviour of this metabolite at the bare glassy carbon electrode was found to be well defined by using differential-pulse voltammetry, and a 90% decrease in peak height was observed on binding of the antibody to the antigen, which occurs at the electroactive site of 7-OH-coumarin. The kinetics of the antibody-antigen reaction was investigated and a first-order reaction was observed with k = 0.0329 mA min-1. This system provides a novel method for studying antibody specificity and the kinetics of such antibody-antigen interactions.}, added-at = {2007-11-03T00:18:07.000+0100}, address = {School of Chemical Sciences, Dublin City University, Ireland.}, author = {Dempsey, E. and O'Sullivan, C. and Smyth, M. R. and Egan, D. and O'Kennedy, R. and Wang, J.}, biburl = {https://www.bibsonomy.org/bibtex/28d351573438813ae5312de4c9c311e0a/bsmyth}, citeulike-article-id = {700744}, description = {my barry smyth}, interhash = {94ac8d257f9e3635419be83f9ccbc2ea}, intrahash = {8d351573438813ae5312de4c9c311e0a}, issn = {0003-2654}, journal = {Analyst}, keywords = {amperometry antibody electrochemistry immunoelectrode}, month = {April}, number = 4, pages = {411--413}, priority = {2}, timestamp = {2007-11-03T00:18:13.000+0100}, title = {Development of an antibody-based amperometric biosensor to study the reaction of 7-hydroxycoumarin with its specific antibody.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8494174}, volume = 118, year = 1993 }