%0 Journal Article %1 Macchia2001 %A Macchia, M. %A Salvetti, F. %A Bertini, S. %A Bussolo, V. Di %A Gattuso, L. %A Gesi, M. %A Hamdan, M. %A Klotz, K. N. %A Laragione, T. %A Lucacchini, A. %A Minutolo, F. %A Nencetti, S. %A Papi, C. %A Tuscano, D. %A Martini, C. %D 2001 %J Bioorg Med Chem Lett %K A2A A3 Adenosine Adenosine-5'-(N-ethylcarboxamide)/*chemistry Animals Benzofurans/chemistry CHO Cell Compounds/chemistry Cricetinae Cultured Dyes/*chemistry/*metabolism Fluorescence Fluorescent Humans Ligands Membrane/metabolism Microscopy, Nitro P1/genetics/*metabolism Proteins/genetics/metabolism Purinergic Recombinant Receptor %N 23 %P 3023-6 %T 7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine (NECA) as new fluorescent probes for human A(3) adenosine receptors %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11714602 %V 11 %X New fluorescent ligands for adenosine receptors (ARs), obtained by the insertion, in the N(6) position of NECA, of NBD-moieties with linear alkyl spacers of increasing length, proved to possess a high affinity and selectivity for the A(3) subtype expressed in CHO cells. In fluorescence microscopy assays, compound 2d, the most active and selective for human A(3)-AR, permitted visualization and localization of this human receptor subtype, showing its potential suitability for internalization and trafficking studies in living cells.

@article{Macchia2001, abstract = {New fluorescent ligands for adenosine receptors (ARs), obtained by the insertion, in the N(6) position of NECA, of NBD-moieties with linear alkyl spacers of increasing length, proved to possess a high affinity and selectivity for the A(3) subtype expressed in CHO cells. In fluorescence microscopy assays, compound 2d, the most active and selective for human A(3)-AR, permitted visualization and localization of this human receptor subtype, showing its potential suitability for internalization and trafficking studies in living cells.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Macchia, M. and Salvetti, F. and Bertini, S. and Bussolo, V. Di and Gattuso, L. and Gesi, M. and Hamdan, M. and Klotz, K. N. and Laragione, T. and Lucacchini, A. and Minutolo, F. and Nencetti, S. and Papi, C. and Tuscano, D. and Martini, C.}, biburl = {https://www.bibsonomy.org/bibtex/2baa09e72f79fd0252d92a4cc04ac77e7/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {9606c0528ff0013ba002c7355a2c7d80}, intrahash = {baa09e72f79fd0252d92a4cc04ac77e7}, issn = {0960-894X (Print) 0960-894X (Linking)}, journal = {Bioorg Med Chem Lett}, keywords = {A2A A3 Adenosine Adenosine-5'-(N-ethylcarboxamide)/*chemistry Animals Benzofurans/chemistry CHO Cell Compounds/chemistry Cricetinae Cultured Dyes/*chemistry/*metabolism Fluorescence Fluorescent Humans Ligands Membrane/metabolism Microscopy, Nitro P1/genetics/*metabolism Proteins/genetics/metabolism Purinergic Recombinant Receptor}, month = {Dec 3}, note = {Macchia, M Salvetti, F Bertini, S Di Bussolo, V Gattuso, L Gesi, M Hamdan, M Klotz, K N Laragione, T Lucacchini, A Minutolo, F Nencetti, S Papi, C Tuscano, D Martini, C England Bioorganic \& medicinal chemistry letters Bioorg Med Chem Lett. 2001 Dec 3;11(23):3023-6.}, number = 23, pages = {3023-6}, shorttitle = {7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine (NECA) as new fluorescent probes for human A(3) adenosine receptors}, timestamp = {2010-12-14T18:20:50.000+0100}, title = {7-Nitrobenzofurazan (NBD) derivatives of 5'-N-ethylcarboxamidoadenosine (NECA) as new fluorescent probes for human A(3) adenosine receptors}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11714602}, volume = 11, year = 2001 }