%0 Journal Article %1 Frosch.20070920 %A Frosch, Torsten %A Koncarevic, Sasa %A Zedler, Linda %A Schmitt, Michael %A Schenzel, Karla %A Becker, Katja %A Popp, J�rgen %D 2007/09/20/ %J J Phys Chem B %K Animals Erythrocytes Hemeproteins Humans IFZ Malaria Molecular_Structure Plasmodium_falciparum Raman Spectrum_Analysis Trophozoites %N 37 %P 11047-11056 %T In situ localization and structural analysis of the malaria pigment hemozoin %V 111 %X Raman microspectroscopy was applied for an in situ localization of the malaria pigment hemozoin in Plasmodium falciparum-infected erythrocytes. The Raman spectra (lambdaexc=633 nm) of hemozoin show very intense signals with a very good signal-to-noise ratio. These in situ Raman signals of hemozoin were compared to Raman spectra of extracted hemozoin, of the synthetic analogue beta-hematin, and of hematin and hemin. beta-Hematin was synthesized according to the acid-catalyzed dehydration of hematin and the anhydrous dehydrohalogenation of hemin which lead to good crystals with lengths of about 5-30 microm. The Raman spectra (lambdaexc=1064 nm) of hemozoin and beta-hematin show almost identical behaviors, while some low wavenumber modes might be used to distinguish between the morphology of differently synthesized beta-hematin samples. The intensity pattern of the resonance Raman spectra (lambdaexc=568 nm) of hemozoin and beta-hematin differ significantly from those of hematin and hemin.

@article{Frosch.20070920, abstract = {Raman microspectroscopy was applied for an in situ localization of the malaria pigment hemozoin in Plasmodium falciparum-infected erythrocytes. The Raman spectra (lambdaexc=633 nm) of hemozoin show very intense signals with a very good signal-to-noise ratio. These in situ Raman signals of hemozoin were compared to Raman spectra of extracted hemozoin, of the synthetic analogue beta-hematin, and of hematin and hemin. beta-Hematin was synthesized according to the acid-catalyzed dehydration of hematin and the anhydrous dehydrohalogenation of hemin which lead to good crystals with lengths of about 5-30 microm. The Raman spectra (lambdaexc=1064 nm) of hemozoin and beta-hematin show almost identical behaviors, while some low wavenumber modes might be used to distinguish between the morphology of differently synthesized beta-hematin samples. The intensity pattern of the resonance Raman spectra (lambdaexc=568 nm) of hemozoin and beta-hematin differ significantly from those of hematin and hemin.}, added-at = {2008-10-14T16:07:18.000+0200}, author = {Frosch, Torsten and Koncarevic, Sasa and Zedler, Linda and Schmitt, Michael and Schenzel, Karla and Becker, Katja and Popp, J�rgen}, biburl = {https://www.bibsonomy.org/bibtex/2ca1c6a601bdcd01ec74326eee3a78393/nutribiochem}, interhash = {9b5f64ac3d03bbcedf196bf3b99af632}, intrahash = {ca1c6a601bdcd01ec74326eee3a78393}, issn = {1520-6106}, journal = {J Phys Chem B}, keywords = {Animals Erythrocytes Hemeproteins Humans IFZ Malaria Molecular_Structure Plasmodium_falciparum Raman Spectrum_Analysis Trophozoites}, number = 37, pages = {11047-11056}, timestamp = {2008-10-14T16:08:41.000+0200}, title = {In situ localization and structural analysis of the malaria pigment hemozoin}, volume = 111, year = {2007/09/20/} }