%0 Journal Article %1 Huisken:2009:Development:19465594 %A Huisken, J %A Stainier, D Y %D 2009 %J Development %K biology cells development embryos microscopy %N 12 %P 1963-1975 %R 10.1242/dev.022426 %T Selective plane illumination microscopy techniques in developmental biology %U http://www.ncbi.nlm.nih.gov/pubmed/19465594 %V 136 %X Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.

@article{Huisken:2009:Development:19465594, abstract = {Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.}, added-at = {2014-09-09T18:13:36.000+0200}, author = {Huisken, J and Stainier, D Y}, biburl = {https://www.bibsonomy.org/bibtex/24ad3c02932185c987060198eb52f3219/alex_ruff}, description = {Selective plane illumination microscopy techniqu... [Development. 2009] - PubMed - NCBI}, doi = {10.1242/dev.022426}, interhash = {9f1d9fcfb02f64404f24ccf011e05ae3}, intrahash = {4ad3c02932185c987060198eb52f3219}, journal = {Development}, keywords = {biology cells development embryos microscopy}, month = jun, number = 12, pages = {1963-1975}, pmid = {19465594}, timestamp = {2014-09-09T18:13:36.000+0200}, title = {Selective plane illumination microscopy techniques in developmental biology}, url = {http://www.ncbi.nlm.nih.gov/pubmed/19465594}, volume = 136, year = 2009 }