%0 Journal Article %1 Dong2016 %A Dong, Biqin %A Almassalha, Luay %A Urban, Ben E. %A Nguyen, The Quyen %A Khuon, Satya %A Chew, Teng Leong %A Backman, Vadim %A Sun, Cheng %A Zhang, Hao F. %D 2016 %I Nature Publishing Group %J Nature Communications %K Fluorescence emission microscopy spectral storm superresolution %N 1 %P 1--8 %R 10.1038/ncomms12290 %T Super-resolution spectroscopic microscopy via photon localization %V 7 %X Traditional photon localization microscopy analyses only the spatial distributions of photons emitted by individual molecules to reconstruct super-resolution optical images. Unfortunately, however, the highly valuable spectroscopic information from these photons have been overlooked. Here we report a spectroscopic photon localization microscopy that is capable of capturing the inherent spectroscopic signatures of photons from individual stochastic radiation events. Spectroscopic photon localization microscopy achieved higher spatial resolution than traditional photon localization microscopy through spectral discrimination to identify the photons emitted from individual molecules. As a result, we resolved two fluorescent molecules, which were 15 nm apart, with the corresponding spatial resolution of 10 nm-a four-fold improvement over photon localization microscopy. Using spectroscopic photon localization microscopy, we further demonstrated simultaneous multi-colour super-resolution imaging of microtubules and mitochondria in COS-7 cells and showed that background autofluorescence can be identified through its distinct emission spectra.

@article{Dong2016, abstract = {Traditional photon localization microscopy analyses only the spatial distributions of photons emitted by individual molecules to reconstruct super-resolution optical images. Unfortunately, however, the highly valuable spectroscopic information from these photons have been overlooked. Here we report a spectroscopic photon localization microscopy that is capable of capturing the inherent spectroscopic signatures of photons from individual stochastic radiation events. Spectroscopic photon localization microscopy achieved higher spatial resolution than traditional photon localization microscopy through spectral discrimination to identify the photons emitted from individual molecules. As a result, we resolved two fluorescent molecules, which were 15 nm apart, with the corresponding spatial resolution of 10 nm-a four-fold improvement over photon localization microscopy. Using spectroscopic photon localization microscopy, we further demonstrated simultaneous multi-colour super-resolution imaging of microtubules and mitochondria in COS-7 cells and showed that background autofluorescence can be identified through its distinct emission spectra.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Dong, Biqin and Almassalha, Luay and Urban, Ben E. and Nguyen, The Quyen and Khuon, Satya and Chew, Teng Leong and Backman, Vadim and Sun, Cheng and Zhang, Hao F.}, biburl = {https://www.bibsonomy.org/bibtex/2fddac037aa420009cecf8026824782c3/kfriedl}, doi = {10.1038/ncomms12290}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Dong et al. - 2016 - Super-resolution spectroscopic microscopy via photon localization.pdf:pdf}, interhash = {9fd8594b7a1577268563caa356f96c5c}, intrahash = {fddac037aa420009cecf8026824782c3}, issn = {20411723}, journal = {Nature Communications}, keywords = {Fluorescence emission microscopy spectral storm superresolution}, month = jul, number = 1, pages = {1--8}, publisher = {Nature Publishing Group}, timestamp = {2020-03-23T22:25:34.000+0100}, title = {{Super-resolution spectroscopic microscopy via photon localization}}, volume = 7, year = 2016 }