%0 Journal Article %1 Jacobs2010 %A Jacobs, S. %A Calebiro, D. %A Nikolaev, V. O. %A Lohse, M. J. %A Schulz, S. %D 2010 %J Endocrinology %K AMP/*metabolism Animals Cultured Cyclic Dynamics Energy Exchange Factors Factors/genetics/metabolism Female Fluorescence G-Protein-Coupled/genetics/metabolism Gland/cytology/*metabolism Guanine Knockout Luminescent Male Mice Molecular Nucleotide Pituitary Proteins/genetics/metabolism Resonance Signal Simulation Somatostatin/genetics/*metabolism Time Transduction Transfer/methods Transgenic Receptor Cell %N 9 %P 4560-5 %T Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary %U http://www.ncbi.nlm.nih.gov/pubmed/20610560 %V 151 %X Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains challenging. Here, we describe the use of transgenic mice for cAMP imaging in living pituitary slices and primary pituitary cells. This technique can be widely used to assess the contribution of various pituitary receptors, including individual G(i) protein-coupled somatostatin receptors, to the regulation of cAMP levels under physiologically relevant settings.

@article{Jacobs2010, abstract = {Fluorescence resonance energy transfer using genetically encoded biosensors has proven to be a powerful technique to monitor the spatiotemporal dynamics of cAMP signals stimulated by G(s)-coupled receptors in living cells. In contrast, real-time imaging of G(i)-mediated cAMP signals under native conditions remains challenging. Here, we describe the use of transgenic mice for cAMP imaging in living pituitary slices and primary pituitary cells. This technique can be widely used to assess the contribution of various pituitary receptors, including individual G(i) protein-coupled somatostatin receptors, to the regulation of cAMP levels under physiologically relevant settings.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Jacobs, S. and Calebiro, D. and Nikolaev, V. O. and Lohse, M. J. and Schulz, S.}, biburl = {https://www.bibsonomy.org/bibtex/2c05e24909b3038fe5f8dedffa6e691c7/pharmawuerz}, endnotereftype = {Journal Article}, groups = {private}, interhash = {a21af23cb50500e8ca65cb55e3eac601}, intrahash = {c05e24909b3038fe5f8dedffa6e691c7}, issn = {1945-7170 (Electronic) 0013-7227 (Linking)}, journal = {Endocrinology}, keywords = {AMP/*metabolism Animals Cultured Cyclic Dynamics Energy Exchange Factors Factors/genetics/metabolism Female Fluorescence G-Protein-Coupled/genetics/metabolism Gland/cytology/*metabolism Guanine Knockout Luminescent Male Mice Molecular Nucleotide Pituitary Proteins/genetics/metabolism Resonance Signal Simulation Somatostatin/genetics/*metabolism Time Transduction Transfer/methods Transgenic Receptor Cell}, month = Sep, note = {Jacobs, Stefan Calebiro, Davide Nikolaev, Viacheslav O Lohse, Martin J Schulz, Stefan United States Endocrinology Endocrinology. 2010 Sep;151(9):4560-5. Epub 2010 Jul 7.}, number = 9, pages = {4560-5}, shorttitle = {Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary}, timestamp = {2010-12-14T18:21:25.000+0100}, title = {Real-time monitoring of somatostatin receptor-cAMP signaling in live pituitary}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20610560}, volume = 151, year = 2010 }