%0 Journal Article %1 RN232 %A Toral, M.I. %A Sabay, T. %A Orellana, S.L. %A Richter, P. %D 2015 %J Journal of Aoac International %K antibiotics, aquaculture, detection, dqcauchile fluorescence liquid-chromatography, performance residues, samples tetracycline %N 3 %P 559-565 %R 10.5740/jaoacint.14-027 %T Determination of Oxytetracycline from Salmon Muscle and Skin by Derivative Spectrophotometry %U /brokenurl#<Go to ISI>://WOS:000356903400004 %V 98 %X A method was developed for the identification and quantification of oxytetracycline residues present in salmon muscle and skin using UV-Vis derivative spectrophotometry. With this method, it was possible to reduce the number of steps in the procedure typically required for instrumental analysis of a sample. The spectral variables, order of the derivative, scale factor, smoothing factor, and analytical wavelength were optimized using standard solutions of oxytetracycline dissolved in 900 mg/L oxalic acid in methanol. The matrix effect was significant; therefore, quantification for oxytetracycline residues was carried out using drug-free salmon muscle and skin samples fortified with oxytetracycline. The LOD and LOQ were found to be 271 and 903 mu g/kg, respectively. The precision and accuracy of the method were validated using drug-free salmon muscle and skin tissues fortified at three different concentrations (8, 16, and 32 mg/kg) on 3 different days. The recoveries at all fortified concentrations were between 90 and 105%, and RSDs in all cases were less than 6.5%. This method can be used to screen out compliant samples and thereby reduce the number of suspect positive samples that will require further confirmatory analysis.

@article{RN232, abstract = {A method was developed for the identification and quantification of oxytetracycline residues present in salmon muscle and skin using UV-Vis derivative spectrophotometry. With this method, it was possible to reduce the number of steps in the procedure typically required for instrumental analysis of a sample. The spectral variables, order of the derivative, scale factor, smoothing factor, and analytical wavelength were optimized using standard solutions of oxytetracycline dissolved in 900 mg/L oxalic acid in methanol. The matrix effect was significant; therefore, quantification for oxytetracycline residues was carried out using drug-free salmon muscle and skin samples fortified with oxytetracycline. The LOD and LOQ were found to be 271 and 903 mu g/kg, respectively. The precision and accuracy of the method were validated using drug-free salmon muscle and skin tissues fortified at three different concentrations (8, 16, and 32 mg/kg) on 3 different days. The recoveries at all fortified concentrations were between 90 and 105%, and RSDs in all cases were less than 6.5%. This method can be used to screen out compliant samples and thereby reduce the number of suspect positive samples that will require further confirmatory analysis.}, added-at = {2019-12-04T03:57:35.000+0100}, author = {Toral, M.I. and Sabay, T. and Orellana, S.L. and Richter, P.}, biburl = {https://www.bibsonomy.org/bibtex/2e11cf569da4abb79d796b4ad66d18ff9/dqcauchile}, doi = {10.5740/jaoacint.14-027}, interhash = {ab5046823e0b27734656e7889edd9ebe}, intrahash = {e11cf569da4abb79d796b4ad66d18ff9}, issn = {1060-3271}, journal = {Journal of Aoac International}, keywords = {antibiotics, aquaculture, detection, dqcauchile fluorescence liquid-chromatography, performance residues, samples tetracycline}, number = 3, pages = {559-565}, timestamp = {2019-12-04T03:58:17.000+0100}, title = {Determination of Oxytetracycline from Salmon Muscle and Skin by Derivative Spectrophotometry}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000356903400004}, volume = 98, year = 2015 }