%0 Journal Article %1 xu_rapid_2009 %A Xu, Xiangrong %A Koetzner, Lee %A Boulet, Jamie %A Maselli, Harry %A Beyenhof, Jessica %A Grover, Gary %D 2009 %J Biomedical Chromatography: BMC %K Acid, Animals, Aspirin, Calibration, Chromatography, Dogs, Drug Linear Liquid, Male, Mass Models, Pharmacokinetics, Rats, Reproducibility Results, Salicylic Sensitivity Specificity, Spectrometry Stability, Tandem and of {Sprague-Dawley,} %N 9 %P 973--979 %R 10.1002/bmc.1209 %T Rapid and sensitive determination of acetylsalicylic acid and salicylic acid in plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study %U http://www.ncbi.nlm.nih.gov/pubmed/19358313 %V 23 %X A simple and sensitive analytical method using liquid chromatography-tandem mass spectrometry (LC/MS/MS) for determination of acetylsalicylic acid (aspirin, ASA) and its major metabolite, salicylic acid (SA), in animal plasma has been developed and validated. Both ASA and SA in plasma samples containing potassium fluoride were extracted using acetonitrile (protein precipitation) with 0.1\% formic acid in it. 6-Methoxysalicylic acid was used as the internal standard (IS). The compounds were separated on a reversed-phase column. The multiple reaction monitoring mode was used with ion transitions of m/z 178.9 --\textgreater 136.8, 137.0 --\textgreater 93.0 and 167.0 --\textgreater 123.0 for ASA, SA and IS, respectively. The lower limits of quantification for ASA and SA were 3 and 30 ng/mL, respectively. The developed method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after p.o. and i.v. administration of 1 mg/kg to rats.

@article{xu_rapid_2009, abstract = {A simple and sensitive analytical method using liquid chromatography-tandem mass spectrometry {(LC/MS/MS)} for determination of acetylsalicylic acid (aspirin, {ASA)} and its major metabolite, salicylic acid {(SA),} in animal plasma has been developed and validated. Both {ASA} and {SA} in plasma samples containing potassium fluoride were extracted using acetonitrile (protein precipitation) with 0.1\% formic acid in it. {6-Methoxysalicylic} acid was used as the internal standard {(IS).} The compounds were separated on a reversed-phase column. The multiple reaction monitoring mode was used with ion transitions of m/z 178.9 --{\textgreater} 136.8, 137.0 --{\textgreater} 93.0 and 167.0 --{\textgreater} 123.0 for {ASA,} {SA} and {IS,} respectively. The lower limits of quantification for {ASA} and {SA} were 3 and 30 {ng/mL,} respectively. The developed method was successfully applied for the evaluation of pharmacokinetics of {ASA} and {SA} after p.o. and i.v. administration of 1 mg/kg to rats.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Xu, Xiangrong and Koetzner, Lee and Boulet, Jamie and Maselli, Harry and Beyenhof, Jessica and Grover, Gary}, biburl = {https://www.bibsonomy.org/bibtex/2691c5f00c0fe55d72d8c49fdfc6e5a85/jelias}, doi = {10.1002/bmc.1209}, interhash = {ae66a7c8bd9ca0525b7f127933806630}, intrahash = {691c5f00c0fe55d72d8c49fdfc6e5a85}, issn = {1099-0801}, journal = {Biomedical Chromatography: {BMC}}, keywords = {Acid, Animals, Aspirin, Calibration, Chromatography, Dogs, Drug Linear Liquid, Male, Mass Models, Pharmacokinetics, Rats, Reproducibility Results, Salicylic Sensitivity Specificity, Spectrometry Stability, Tandem and of {Sprague-Dawley,}}, month = sep, note = {{PMID:} 19358313}, number = 9, pages = {973--979}, shorttitle = {Rapid and sensitive determination of acetylsalicylic acid and salicylic acid in plasma using liquid chromatography-tandem mass spectrometry}, timestamp = {2011-03-11T10:05:46.000+0100}, title = {Rapid and sensitive determination of acetylsalicylic acid and salicylic acid in plasma using liquid chromatography-tandem mass spectrometry: application to pharmacokinetic study}, url = {http://www.ncbi.nlm.nih.gov/pubmed/19358313}, volume = 23, year = 2009 }