%0 Journal Article %1 citeulike:467042 %A Ishimori, Y. %A Yasuda, T. %A Tsumita, T. %A Notsuki, M. %A Koyama, M. %A Tadakuma, T. %D 1984 %J J Immunol Methods %K l-i-l-a ab_detection complement immunoassay homogeneous liposome %N 2 %P 351--360 %T Liposome immune lysis assay (LILA): a simple method to measure anti-protein antibody using protein antigen-bearing liposomes. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6549183 %V 75 %X A new simple immunoassay technique using immune lysis of liposomes was developed to measure antibody against protein antigens. Multilamellar liposomes were composed of dipalmitoylphosphatidylcholine, cholesterol and phosphatidylethanolamine substituted with the hetero-bifunctional cross-linking reagent N-hydroxysuccinimidyl 3-(2-pyridyldithio)propionate (SPDP). The protein antigen (human IgG) was coupled to these liposomes after treatment with SPDP and mild reduction. As a release marker, carboxyfluorescein (CF) was entrapped in the liposomes. The CF release was specific to anti-human IgG antibody and depended on the presence of complement. This technique could detect 10(-15) mol of anti-human IgG antibody or human IgG. The liposomes were stable over 8 months at 4 degrees C under nitrogen gas.

@article{citeulike:467042, abstract = {A new simple immunoassay technique using immune lysis of liposomes was developed to measure antibody against protein antigens. Multilamellar liposomes were composed of dipalmitoylphosphatidylcholine, cholesterol and phosphatidylethanolamine substituted with the hetero-bifunctional cross-linking reagent N-hydroxysuccinimidyl 3-(2-pyridyldithio)propionate (SPDP). The protein antigen (human IgG) was coupled to these liposomes after treatment with SPDP and mild reduction. As a release marker, carboxyfluorescein (CF) was entrapped in the liposomes. The CF release was specific to anti-human IgG antibody and depended on the presence of complement. This technique could detect 10(-15) mol of anti-human IgG antibody or human IgG. The liposomes were stable over 8 months at 4 degrees C under nitrogen gas.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Ishimori, Y. and Yasuda, T. and Tsumita, T. and Notsuki, M. and Koyama, M. and Tadakuma, T.}, biburl = {https://www.bibsonomy.org/bibtex/24aefa17c366012ad5ae631e4f6a6a669/biblio24}, citeulike-article-id = {467042}, interhash = {b12081ca53e947aa3e9c05d5b261175c}, intrahash = {4aefa17c366012ad5ae631e4f6a6a669}, issn = {0022-1759}, journal = {J Immunol Methods}, keywords = {l-i-l-a ab_detection complement immunoassay homogeneous liposome}, month = {December}, number = 2, pages = {351--360}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Liposome immune lysis assay (LILA): a simple method to measure anti-protein antibody using protein antigen-bearing liposomes.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=6549183}, volume = 75, year = 1984 }