%0 Journal Article %1 Elli_2001_114 %A Ellis, R. J. %D 2001 %J Curr. Opin. Struct. Biol. %K , 80 AIDS Acids, Active, Adenocarcinoma, Adolescent, Adult, Aged, Analysis Analysis, Animals, Antagonists, Antibodies, Antiretroviral Arousal, Atrophy, Bacteria, Bacterial, Biological Biological, Biopsy, Brain, Care, Cells, Chaperones, Chaperonins, Chemical, Cognition Cohort Combined Comparative Complex, Concentration, Conformal, Copper, Corpus Cross-Sectional Cyanide, Cyclopropanes, Cytoplasm, DNA, Dementia Diffusion, Disorders, Double-Blind Escherichia Eukaryotic Fatty Female, Fluorescence, Genotype, HIV HIV-1, Health Heavy, Highly Humans, Hydrogen Hydrogen-Ion Imaging, In Indium Infections, Intraoperative Kinetics, Limbic Macromolecular Magnetic Male, Markers, Metals, Method, Middle Mobile Modality Models, Molecular Monoclonal, Multivariate Naltrexone, Narcotic Nematoda, Neoplasms, Period, Radioisotopes, Resonance Seropositivity, Striatum, Studies, Study, Substances, System, Therapy, Units, Variance, Vitro, and coli, of over, %N 1 %P 114--119 %T Macromolecular crowding: an important but neglected aspect of the intracellular environment. %U http://dx.doi.org/10.1016/S0959-440X(00)00172-X %V 11 %X Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and composition, redox potential and the concentrations of relevant metabolites and effector molecules. There is one universal aspect of the cellular interior, however, that is largely neglected--the fact that it is highly crowded with macromolecules. It is proposed that the addition of crowding agents should become as routine as controlling pH and ionic strength if we are to meet the objective of studying biological molecules under more physiologically relevant conditions.

@article{Elli_2001_114, abstract = {Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and composition, redox potential and the concentrations of relevant metabolites and effector molecules. There is one universal aspect of the cellular interior, however, that is largely neglected--the fact that it is highly crowded with macromolecules. It is proposed that the addition of crowding agents should become as routine as controlling pH and ionic strength if we are to meet the objective of studying biological molecules under more physiologically relevant conditions.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Ellis, R. J.}, biburl = {https://www.bibsonomy.org/bibtex/2f57de1ca1a346b7026d5cfc17fe1a0df/hake}, description = {The whole bibliography file I use.}, file = {Elli_2001_114.pdf:Elli_2001_114.pdf:PDF}, interhash = {c168b38e396791238c4b1196ed165987}, intrahash = {f57de1ca1a346b7026d5cfc17fe1a0df}, journal = {Curr. Opin. Struct. Biol.}, key = 176, keywords = {, 80 AIDS Acids, Active, Adenocarcinoma, Adolescent, Adult, Aged, Analysis Analysis, Animals, Antagonists, Antibodies, Antiretroviral Arousal, Atrophy, Bacteria, Bacterial, Biological Biological, Biopsy, Brain, Care, Cells, Chaperones, Chaperonins, Chemical, Cognition Cohort Combined Comparative Complex, Concentration, Conformal, Copper, Corpus Cross-Sectional Cyanide, Cyclopropanes, Cytoplasm, DNA, Dementia Diffusion, Disorders, Double-Blind Escherichia Eukaryotic Fatty Female, Fluorescence, Genotype, HIV HIV-1, Health Heavy, Highly Humans, Hydrogen Hydrogen-Ion Imaging, In Indium Infections, Intraoperative Kinetics, Limbic Macromolecular Magnetic Male, Markers, Metals, Method, Middle Mobile Modality Models, Molecular Monoclonal, Multivariate Naltrexone, Narcotic Nematoda, Neoplasms, Period, Radioisotopes, Resonance Seropositivity, Striatum, Studies, Study, Substances, System, Therapy, Units, Variance, Vitro, and coli, of over,}, month = Feb, number = 1, pages = {114--119}, pii = {S0959440X0000172X}, pmid = {11179900}, timestamp = {2009-06-03T11:21:10.000+0200}, title = {Macromolecular crowding: an important but neglected aspect of the intracellular environment.}, url = {http://dx.doi.org/10.1016/S0959-440X(00)00172-X}, volume = 11, year = 2001 }