%0 Journal Article %1 Gao2010Rational %A Gao, Hong %A Zhou, Xianlong %A Gou, Zhongxuan %A Zhuo, Ying %A Fu, Chengzhang %A Liu, Mei %A Song, Fuhang %A Ashforth, Elizabeth %A Zhang, Lixin %D 2010 %J Antonie van Leeuwenhoek %K metabolic-engineering strain-improvement %R 10.1007/s10482-010-9442-4 %T Rational design for over-production of desirable microbial metabolites by precision engineering. %U http://dx.doi.org/10.1007/s10482-010-9442-4 %X Microbes represent a valuable source of commercially significant natural products that have improved our quality of life. Precision engineering can be used to precisely identify and specifically modify genes responsible for production of natural products and improve this production or modify the genes creating products that would not otherwise be produced. There have been several success stories concerning the manipulation of regulatory genes, pathways, and genomes to increase the productivity of industrial microbes. This review will focus on the strategies and integrated approaches for precisely deciphering regulatory genes by various modern techniques. The applications of precision engineering in rational strain improvement also shed light on the biology of natural microbial systems.

@article{Gao2010Rational, abstract = {Microbes represent a valuable source of commercially significant natural products that have improved our quality of life. Precision engineering can be used to precisely identify and specifically modify genes responsible for production of natural products and improve this production or modify the genes creating products that would not otherwise be produced. There have been several success stories concerning the manipulation of regulatory genes, pathways, and genomes to increase the productivity of industrial microbes. This review will focus on the strategies and integrated approaches for precisely deciphering regulatory genes by various modern techniques. The applications of precision engineering in rational strain improvement also shed light on the biology of natural microbial systems.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Gao, Hong and Zhou, Xianlong and Gou, Zhongxuan and Zhuo, Ying and Fu, Chengzhang and Liu, Mei and Song, Fuhang and Ashforth, Elizabeth and Zhang, Lixin}, biburl = {https://www.bibsonomy.org/bibtex/28558e7fb4c4cd0e5e341dd15f494edac/karthikraman}, citeulike-article-id = {7046634}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10482-010-9442-4}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/20401739}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=20401739}, day = 18, doi = {10.1007/s10482-010-9442-4}, interhash = {caa4f46a17c475ed49589f7ff3b543f8}, intrahash = {8558e7fb4c4cd0e5e341dd15f494edac}, issn = {1572-9699}, journal = {Antonie van Leeuwenhoek}, keywords = {metabolic-engineering strain-improvement}, month = apr, pmid = {20401739}, posted-at = {2010-04-21 08:34:59}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Rational design for over-production of desirable microbial metabolites by precision engineering.}, url = {http://dx.doi.org/10.1007/s10482-010-9442-4}, year = 2010 }