%0 Journal Article %1 Luky_1998_667 %A Lukyanenko, V. %A Wiesner, T. F. %A Gyorke, S. %D 1998 %J J. Physiol. %K 9508828 Algorithms, Aniline Animals, Calcium Calcium, Cells, Channels, Chemical, Compounds, Confocal, Cultured, Dyes, Fluorescent Gov't, Heart Heart, Kinetics, Microscopy, Models, Non-U.S. P.H.S., Rats, Reproducibility Research Results, Reticulum, Sarcoplasmic Sprague-Dawley, Support, Tacrolimus, U.S. Ventricles, Xanthenes, of %P 667--677 %T Termination of Ca$^2+$ release during Ca$^2+$ sparks in rat ventricular myocytes. %U http://jp.physoc.org/cgi/content/full/507/3/667 %V 507 ( Pt 3) %X 1. Confocal Ca$^2+$ imaging was used to measure spontaneous release events (Ca$^2+$ sparks) in fluo-3-loaded isolated rat ventricular myocytes. 2. The microscopic Ca$^2+$ release flux underlying Ca$^2+$ sparks was derived by adapting the methods used previously to describe macroscopic Ca$^2+$ release from cell-averaged Ca$^2+$ transients. 3. The magnitude of the local release fluxes varied from 2 to 5 microM ms-1, depending on SR Ca$^2+$ loading conditions. Following spontaneous activation, the release flux rapidly decayed (tau = 6-12 ms). The rate of termination of release flux was found to be directly related to the magnitude of the flux (r2 = 0.88). 4. The rate of termination of local release flux was slowed in the presence of FK506, a compound that is known to reduce inactivation of SR Ca$^2+$ channels in vitro. 5. These results suggest that termination of release flux during sparks is not due to a spontaneous stochastic decay process or local depletion of Ca$^2+$ from the SR, but rather involves an active extinguishing mechanism such as Ca$^2+$-dependent inactivation or adaptation.

@article{Luky_1998_667, abstract = {1. Confocal {C}a$^{2+}$ imaging was used to measure spontaneous release events ({C}a$^{2+}$ sparks) in fluo-3-loaded isolated rat ventricular myocytes. 2. The microscopic {C}a$^{2+}$ release flux underlying {C}a$^{2+}$ sparks was derived by adapting the methods used previously to describe macroscopic {C}a$^{2+}$ release from cell-averaged {C}a$^{2+}$ transients. 3. The magnitude of the local release fluxes varied from 2 to 5 microM ms-1, depending on SR {C}a$^{2+}$ loading conditions. Following spontaneous activation, the release flux rapidly decayed (tau = 6-12 ms). The rate of termination of release flux was found to be directly related to the magnitude of the flux (r2 = 0.88). 4. The rate of termination of local release flux was slowed in the presence of FK506, a compound that is known to reduce inactivation of SR {C}a$^{2+}$ channels in vitro. 5. These results suggest that termination of release flux during sparks is not due to a spontaneous stochastic decay process or local depletion of {C}a$^{2+}$ from the SR, but rather involves an active extinguishing mechanism such as {C}a$^{2+}$-dependent inactivation or adaptation.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Lukyanenko, V. and Wiesner, T. F. and Gyorke, S.}, biburl = {https://www.bibsonomy.org/bibtex/2dba782ab2180c6cf6a58ad5204ad0ca5/hake}, description = {The whole bibliography file I use.}, file = {Luky_1998_667.pdf:Luky_1998_667.pdf:PDF}, interhash = {dd1d33270aded21b6c0427af1ece19ec}, intrahash = {dba782ab2180c6cf6a58ad5204ad0ca5}, journal = {J. Physiol.}, keywords = {9508828 Algorithms, Aniline Animals, Calcium Calcium, Cells, Channels, Chemical, Compounds, Confocal, Cultured, Dyes, Fluorescent Gov't, Heart Heart, Kinetics, Microscopy, Models, Non-U.S. P.H.S., Rats, Reproducibility Research Results, Reticulum, Sarcoplasmic Sprague-Dawley, Support, Tacrolimus, U.S. Ventricles, Xanthenes, of}, month = Mar, pages = {667--677}, pmid = {9508828}, timestamp = {2009-06-03T11:21:21.000+0200}, title = {Termination of {C}a$^{2+}$ release during {C}a$^{2+}$ sparks in rat ventricular myocytes.}, url = {http://jp.physoc.org/cgi/content/full/507/3/667}, volume = {507 ( Pt 3)}, year = 1998 }