%0 Journal Article %1 Love:2014:Genome-Biol:25516281 %A Love, M I %A Huber, W %A Anders, S %D 2014 %J Genome Biol %K %N 12 %P 550-550 %R 10.1186/s13059-014-0550-8 %T Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 %U https://www.ncbi.nlm.nih.gov/pubmed/25516281 %V 15 %X In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. We present DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. The DESeq2 package is available at http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html webcite.

@article{Love:2014:Genome-Biol:25516281, abstract = {In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. We present DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. The DESeq2 package is available at http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html webcite.}, added-at = {2018-10-17T21:54:32.000+0200}, author = {Love, M I and Huber, W and Anders, S}, biburl = {https://www.bibsonomy.org/bibtex/20499d28f9c10ed63559a923531f4b1bb/marcsaric}, doi = {10.1186/s13059-014-0550-8}, interhash = {ddf26c9817be0b82f9a256ce81415f69}, intrahash = {0499d28f9c10ed63559a923531f4b1bb}, journal = {Genome Biol}, keywords = {}, number = 12, pages = {550-550}, pmid = {25516281}, timestamp = {2018-10-17T21:54:32.000+0200}, title = {Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2}, url = {https://www.ncbi.nlm.nih.gov/pubmed/25516281}, volume = 15, year = 2014 }