%0 Journal Article %1 lam_new_2011 %A Lâm, Thiên-Trí %A Elias, Johannes %A Frosch, Matthias %A Vogel, Ulrich %A Claus, Heike %D 2011 %J International Journal of Medical Microbiology: IJMM %K Analysis, Bacterial Bacterial, Bacteriological Capsules, Chain Data, False Haemophilus Humans, Infections, Insertional, Molecular Mutagenesis, Negative Polymerase Primers, Reaction, Reactions, Sensitivity Sequence Specificity, Techniques, and bacterial_capsules influenzae, je molecular_epidemiology {DNA} {DNA}, %N 2 %P 176--179 %R 10.1016/j.ijmm.2010.07.004 %T New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142 %U http://www.ncbi.nlm.nih.gov/pubmed/20940106 %V 301 %X A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.

@article{lam_new_2011, abstract = {A new {PCR} protocol for molecular typing of Haemophilus influenzae serotype e {(Hie)} was developed. To this end, the sequence of the cap region {II} of Hie strain {ATCC8142} was identified, which was {\textgreater}99\% identical to the recently published sequence of Hie isolate 274. The {PCR} using primer pair {TTL63/TTL64} amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all {non-Hie} strains, there were no false positives. False-negative results of the {PCR} proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Lâm, Thiên-Trí and Elias, Johannes and Frosch, Matthias and Vogel, Ulrich and Claus, Heike}, biburl = {https://www.bibsonomy.org/bibtex/20f8240b68762942ac75dc9cd4f62f500/ag_vogel}, doi = {10.1016/j.ijmm.2010.07.004}, interhash = {e098689a78df8c4a5cecbc92a6606f06}, intrahash = {0f8240b68762942ac75dc9cd4f62f500}, issn = {1618-0607}, journal = {International Journal of Medical Microbiology: {IJMM}}, keywords = {Analysis, Bacterial Bacterial, Bacteriological Capsules, Chain Data, False Haemophilus Humans, Infections, Insertional, Molecular Mutagenesis, Negative Polymerase Primers, Reaction, Reactions, Sensitivity Sequence Specificity, Techniques, and bacterial_capsules influenzae, je molecular_epidemiology {DNA} {DNA},}, month = feb, note = {{PMID:} 20940106}, number = 2, pages = {176--179}, timestamp = {2014-02-06T18:31:16.000+0100}, title = {New diagnostic {PCR} for Haemophilus influenzae serotype e based on the cap locus of strain {ATCC} 8142}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20940106}, volume = 301, year = 2011 }