%0 Journal Article %1 Panutsopulos.2005 %A Panutsopulos, D. %A Arvanitis, D. L. %A Tsatsanis, C. %A Papalambros, E. %A Sigala, F. %A Spandidos, D. A. %D 2005 %J J.Vasc.Res. %K 80 A Adult Aged Arteries Artery Cell Cells Coronary Disease Endothelial Epithelial Factor Female Growth Human Humans Immediate-Early Intercellular Laboratories Line Macrophages Male Messenger Middle Monocytes Neuregulin-1 Peptides Proteins RNA Signaling Vascular and blood cells genetics metabolism methods over pathology physiopathology protein response secretion %N 6 %P 463-474 %T Expression of heregulin in human coronary atherosclerotic lesions %U PM:16155362 %V 42 %X BACKGROUND: Endothelial cells, monocytes/macrophages, and vascular smooth muscle cells contribute to the establishment and progression of atherosclerotic lesions by expressing growth and inflammatory factors. The aim of the present study was to determine whether heregulin (HRG) is associated with human coronary artery disease. METHODS: Twenty-six fresh human coronary artery segments were collected at autopsy. Expression of cysteine-rich 61 (CYR61) and VEGF in response to HRG was studied in the human endothelial cell line EA.hy926, and expression of CYR61 and HRG was evaluated in activated macrophages isolated from peripheral blood of healthy donors. RESULTS: We found that HRG was overexpressed at the protein and mRNA level in all lesions analyzed and gradually increased as the stages of the lesions progressed. Expression of HRG was observed in the intima primarily in macrophages. The same specimens were analyzed for the expression of CYR61, an angiogenetic factor regulated by HRG in br

@article{Panutsopulos.2005, abstract = {BACKGROUND: Endothelial cells, monocytes/macrophages, and vascular smooth muscle cells contribute to the establishment and progression of atherosclerotic lesions by expressing growth and inflammatory factors. The aim of the present study was to determine whether heregulin (HRG) is associated with human coronary artery disease. METHODS: Twenty-six fresh human coronary artery segments were collected at autopsy. Expression of cysteine-rich 61 (CYR61) and VEGF in response to HRG was studied in the human endothelial cell line EA.hy926, and expression of CYR61 and HRG was evaluated in activated macrophages isolated from peripheral blood of healthy donors. RESULTS: We found that HRG was overexpressed at the protein and mRNA level in all lesions analyzed and gradually increased as the stages of the lesions progressed. Expression of HRG was observed in the intima primarily in macrophages. The same specimens were analyzed for the expression of CYR61, an angiogenetic factor regulated by HRG in br}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Panutsopulos, D. and Arvanitis, D. L. and Tsatsanis, C. and Papalambros, E. and Sigala, F. and Spandidos, D. A.}, biburl = {https://www.bibsonomy.org/bibtex/268da99870febb32cf06b671fe61b7474/kanefendt}, interhash = {e4c12b6f425d1a18b2793881ea9d11a5}, intrahash = {68da99870febb32cf06b671fe61b7474}, journal = {J.Vasc.Res.}, keywords = {80 A Adult Aged Arteries Artery Cell Cells Coronary Disease Endothelial Epithelial Factor Female Growth Human Humans Immediate-Early Intercellular Laboratories Line Macrophages Male Messenger Middle Monocytes Neuregulin-1 Peptides Proteins RNA Signaling Vascular and blood cells genetics metabolism methods over pathology physiopathology protein response secretion}, number = 6, pages = {463-474}, timestamp = {2010-02-05T11:28:43.000+0100}, title = {Expression of heregulin in human coronary atherosclerotic lesions}, url = {PM:16155362}, volume = 42, year = 2005 }