%0 Journal Article %1 Gregor2010Onset %A Gregor, Thomas %A Fujimoto, Koichi %A Masaki, Noritaka %A Sawai, Satoshi %D 2010 %I American Association for the Advancement of Science %J Science (New York, N.Y.) %K communities complex-systems emergence groupthink social %N 5981 %P 1021--1025 %R 10.1126/science.1183415 %T The onset of collective behavior in social amoebae. %U http://dx.doi.org/10.1126/science.1183415 %V 328 %X In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate (cAMP) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular cAMP. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular cAMP plays a critical role in the onset of collective behavior.

@article{Gregor2010Onset, abstract = {In the social amoebae Dictyostelium discoideum, periodic synthesis and release of extracellular cyclic adenosine 3',5'-monophosphate ({cAMP}) guide cell aggregation and commitment to form fruiting bodies. It is unclear whether these oscillations are an intrinsic property of individual cells or if they exist only as a population-level phenomenon. Here, we showed by live-cell imaging of intact cell populations that pulses originate from a discrete location despite constant exchange of cells to and from the region. In a perfusion chamber, both isolated single cells and cell populations switched from quiescence to rhythmic activity depending on the concentration of extracellular {cAMP}. A quantitative analysis showed that stochastic pulsing of individual cells below the threshold concentration of extracellular {cAMP} plays a critical role in the onset of collective behavior.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Gregor, Thomas and Fujimoto, Koichi and Masaki, Noritaka and Sawai, Satoshi}, biburl = {https://www.bibsonomy.org/bibtex/27ad386f2eaee6d60ea7097373fcb9914/karthikraman}, citeulike-article-id = {7203881}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1183415}, citeulike-linkout-1 = {http://www.sciencemag.org/content/328/5981/1021.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/328/5981/1021.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/328/5981/1021}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/20413456}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=20413456}, day = 21, doi = {10.1126/science.1183415}, interhash = {e86484c7edbf583b09df9cdc0e4e3d49}, intrahash = {7ad386f2eaee6d60ea7097373fcb9914}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {communities complex-systems emergence groupthink social}, month = may, number = 5981, pages = {1021--1025}, pmid = {20413456}, posted-at = {2010-05-21 09:01:33}, priority = {2}, publisher = {American Association for the Advancement of Science}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {The onset of collective behavior in social amoebae.}, url = {http://dx.doi.org/10.1126/science.1183415}, volume = 328, year = 2010 }