%0 Journal Article %1 kim2017engineering %A Kim, Moon-Soo %A Kini, Anu Ganesh %D 2017 %J Molecules and Cells %K 5 background biotechnology zincFingers %N 8 %P 533-541 %R 10.14348/molcells.2017.0139 %T Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research %U http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.0139 %V 40 %X Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.

@article{kim2017engineering, abstract = {Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.}, added-at = {2017-10-05T00:41:23.000+0200}, author = {Kim, Moon-Soo and Kini, Anu Ganesh}, biburl = {https://www.bibsonomy.org/bibtex/2150fd71edae98ab24722c7ad7488ce55/artheibault}, description = {This article discusses engineering of synthetic zinc finger proteins to bind to DNA in order to study it. I used it to get a sense of what zinc fingers were, since I noticed some of the important genes highlighted by Teschendorff et al. were involved with them}, doi = {10.14348/molcells.2017.0139}, interhash = {ea36a16d4830c3befe727cbab3b913fb}, intrahash = {150fd71edae98ab24722c7ad7488ce55}, journal = {Molecules and Cells}, keywords = {5 background biotechnology zincFingers}, month = aug, number = 8, pages = {533-541}, timestamp = {2017-10-25T21:58:45.000+0200}, title = {Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research}, url = {http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.0139}, volume = 40, year = 2017 }