%0 Journal Article %1 abele-horn_molecular_2006 %A Abele-Horn, Marianne %A Vogel, Ulrich %A Klare, Ingo %A Konstabel, Carola %A Trabold, Rene %A Kurihara, Risa %A Witte, Wolfgang %A Kreth, Wolfgang %A Schlegel, Paul-Gerhardt %A Claus, Heike %D 2006 %J Journal of Clinical Microbiology %K Analysis, Bacterial Cross Electrophoresis, Enterococcus, Gel, Humans, Infection, Infections, Minisatellite Repeats, Resistance Sequence Streptococcal Techniques, Typing Vancomycin ag_horn {DNA,} {Pulsed-Field,} %N 11 %P 4009--4013 %R 10.1128/JCM.00195-06 %T Molecular epidemiology of hospital-acquired vancomycin-resistant enterococci %U http://www.ncbi.nlm.nih.gov/pubmed/17005748 %V 44 %X Vancomycin-resistant Enterococcus faecium strains are a significant cause of nosocomial infections in predisposed patients. Multiple-locus variable-number tandem repeat analysis (MLVA) has been validated recently by use of a global strain collection. In this report, we applied MLVA together with multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) to type 14 isolates from three clusters of patients colonized or infected with vancomycin-resistant Enterococcus faecium and another 10 epidemiologically unrelated isolates from the same hospital. The clusters could be distinguished by all three typing methods, which proved to be concordant. PFGE patterns provided the highest resolution. We observed seven sequence types (ST), six MLVA types (MT), and nine distinct ST/MT combinations. The combination of MLST and MLVA may be an alternative to PFGE in hospital epidemiology, providing the benefits of high accuracy, reproducibility, and portability.

@article{abele-horn_molecular_2006, abstract = {Vancomycin-resistant Enterococcus faecium strains are a significant cause of nosocomial infections in predisposed patients. Multiple-locus variable-number tandem repeat analysis {(MLVA)} has been validated recently by use of a global strain collection. In this report, we applied {MLVA} together with multilocus sequence typing {(MLST)} and pulsed-field gel electrophoresis {(PFGE)} to type 14 isolates from three clusters of patients colonized or infected with vancomycin-resistant Enterococcus faecium and another 10 epidemiologically unrelated isolates from the same hospital. The clusters could be distinguished by all three typing methods, which proved to be concordant. {PFGE} patterns provided the highest resolution. We observed seven sequence types {(ST),} six {MLVA} types {(MT),} and nine distinct {ST/MT} combinations. The combination of {MLST} and {MLVA} may be an alternative to {PFGE} in hospital epidemiology, providing the benefits of high accuracy, reproducibility, and portability.}, added-at = {2011-04-07T15:44:20.000+0200}, author = {{Abele-Horn}, Marianne and Vogel, Ulrich and Klare, Ingo and Konstabel, Carola and Trabold, Rene and Kurihara, Risa and Witte, Wolfgang and Kreth, Wolfgang and Schlegel, {Paul-Gerhardt} and Claus, Heike}, biburl = {https://www.bibsonomy.org/bibtex/28fe3050d308ad4bceb9da346e0fc51a8/hymi}, doi = {10.1128/JCM.00195-06}, interhash = {ef5ec15f36db897d158a97d7c7d37043}, intrahash = {8fe3050d308ad4bceb9da346e0fc51a8}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Analysis, Bacterial Cross Electrophoresis, Enterococcus, Gel, Humans, Infection, Infections, Minisatellite Repeats, Resistance Sequence Streptococcal Techniques, Typing Vancomycin ag_horn {DNA,} {Pulsed-Field,}}, month = nov, note = {{PMID:} 17005748}, number = 11, pages = {4009--4013}, timestamp = {2011-04-07T16:38:32.000+0200}, title = {Molecular epidemiology of hospital-acquired vancomycin-resistant enterococci}, url = {http://www.ncbi.nlm.nih.gov/pubmed/17005748}, volume = 44, year = 2006 }