%0 Journal Article %1 petkov2020expression %A Petkov, Stefan %A Bekele, Yonas %A Lakshmikanth, Tadepally %A Hejdeman, Bo %A Zazzi, Maurizio %A Brodin, Petter %A Chiodi, Francesca %D 2020 %J Scientific Reports %K cd4 cd8 %N 1 %P 20425-- %R 10.1038/s41598-020-77433-z %T High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood %U https://doi.org/10.1038/s41598-020-77433-z %V 10 %X Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45high T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45high cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45high T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45high T cells may contribute to their exhaustion.

@article{petkov2020expression, abstract = {Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45high T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45high cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45high T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45high T cells may contribute to their exhaustion.}, added-at = {2020-11-29T23:23:27.000+0100}, author = {Petkov, Stefan and Bekele, Yonas and Lakshmikanth, Tadepally and Hejdeman, Bo and Zazzi, Maurizio and Brodin, Petter and Chiodi, Francesca}, biburl = {https://www.bibsonomy.org/bibtex/2fe585178e042c271ea30278f56171f37/lkanth}, description = {High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood | Scientific Reports}, doi = {10.1038/s41598-020-77433-z}, interhash = {d91aa6b43ec8d9c237a5b252f1521dd0}, intrahash = {fe585178e042c271ea30278f56171f37}, issn = {20452322}, journal = {Scientific Reports}, keywords = {cd4 cd8}, number = 1, pages = {20425--}, refid = {Petkov2020}, timestamp = {2020-11-29T23:23:27.000+0100}, title = {High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood}, url = {https://doi.org/10.1038/s41598-020-77433-z}, volume = 10, year = 2020 }