%0 Journal Article %1 Li07 %A Li, K %A Cai, R %A Dai, B B %A Zhang, X Q %A Wang, H J %A Ge, S F %A Xu, W R %A Lu, J %D 2007 %J Biochem. Biophys. Res. Commun. %K Base_Sequence Binding_Sites Blotting,_Western Carrier_Proteins Chromatin_Immunoprecipitation Gene_Expression_Profiling Gene_Expression_Regulation,_Neoplastic Humans Introns K562_Cells Matrix_Attachment_Region_Binding_Proteins Oligonucleotide_Array_Sequence_Analysis Protein_Binding RNA_Interference Reverse_Transcriptase_Polymerase_Chain_Reaction Transfection %N 1 %P 6-12 %R 10.1016/j.bbrc.2007.01.201 %T SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron. %U http://dx.doi.org/10.1016/j.bbrc.2007.01.201 %V 356 %X Special AT-rich binding protein 1 (SATB1), a cell type-specific nuclear matrix attachment region (MAR) DNA-binding protein, tethers to a specific DNA sequence and regulates gene expression through chromatin remodeling and HDAC (histone deacetylase complex) recruitment. In this study, a SATB1 eukaryotic expression plasmid was transfected into the human erythroleukemia K562 cell line and individual clones that stably over-expressed the SATB1 protein were isolated. Microarray analysis revealed that hundreds of genes were either up- or down-regulated in the SATB1 over-expressing K562 cell lines. One of these was the extra-cellular matrix glycoprotein, SPARC (human secreted protein acidic and rich in cysteine). siRNA knock-down of SATB1 also reduced SPARC expression, which was consistent with elevated SPARC levels in the SATB1 over-expressing cell line. Bioinformatics software Mat-inspector showed that a 17bp DNA sequence in the third intron of SPARC possessed a high potential for SATB1 binding; a finding confirmed by Chromatin immunoprecipitation (ChIP) with anti-SATB1 antibody. Our results show for the first time that forced-expression of SATB1 in K562 cells triggers SPARC up-regulation by binding to a 17bp DNA sequence in the third intron.

@article{Li07, abstract = {Special AT-rich binding protein 1 (SATB1), a cell type-specific nuclear matrix attachment region (MAR) DNA-binding protein, tethers to a specific DNA sequence and regulates gene expression through chromatin remodeling and HDAC (histone deacetylase complex) recruitment. In this study, a SATB1 eukaryotic expression plasmid was transfected into the human erythroleukemia K562 cell line and individual clones that stably over-expressed the SATB1 protein were isolated. Microarray analysis revealed that hundreds of genes were either up- or down-regulated in the SATB1 over-expressing K562 cell lines. One of these was the extra-cellular matrix glycoprotein, SPARC (human secreted protein acidic and rich in cysteine). siRNA knock-down of SATB1 also reduced SPARC expression, which was consistent with elevated SPARC levels in the SATB1 over-expressing cell line. Bioinformatics software Mat-inspector showed that a 17bp DNA sequence in the third intron of SPARC possessed a high potential for SATB1 binding; a finding confirmed by Chromatin immunoprecipitation (ChIP) with anti-SATB1 antibody. Our results show for the first time that forced-expression of SATB1 in K562 cells triggers SPARC up-regulation by binding to a 17bp DNA sequence in the third intron.}, added-at = {2010-01-26T20:35:53.000+0100}, author = {Li, K and Cai, R and Dai, B B and Zhang, X Q and Wang, H J and Ge, S F and Xu, W R and Lu, J}, biburl = {https://www.bibsonomy.org/bibtex/2c757685713e2d46c59963dfced2e2310/denilw}, doi = {10.1016/j.bbrc.2007.01.201}, file = {article:../papers/Transcription Factor Binding/SatB1/SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron.pdf:pdf}, institution = {Department of Biochemistry and Molecular Biology, Medical School of Shanghai Jiao Tong University, Shanghai 200025, PR China.}, interhash = {00c3ed3a4166151f6a6b2aeabdc8832d}, intrahash = {c757685713e2d46c59963dfced2e2310}, journal = {Biochem. Biophys. Res. Commun.}, keywords = {Base_Sequence Binding_Sites Blotting,_Western Carrier_Proteins Chromatin_Immunoprecipitation Gene_Expression_Profiling Gene_Expression_Regulation,_Neoplastic Humans Introns K562_Cells Matrix_Attachment_Region_Binding_Proteins Oligonucleotide_Array_Sequence_Analysis Protein_Binding RNA_Interference Reverse_Transcriptase_Polymerase_Chain_Reaction Transfection}, month = Apr, number = 1, owner = {denilw}, pages = {6-12}, pdf = {C:\Users\denilw\Desktop\Steven Jones Lab\Papers\SatB1\SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron.pdf}, pii = {S0006-291X(07)00017-4}, pmid = {17343824}, timestamp = {2010-01-26T20:36:00.000+0100}, title = {SATB1 regulates SPARC expression in K562 cell line through binding to a specific sequence in the third intron.}, url = {http://dx.doi.org/10.1016/j.bbrc.2007.01.201}, volume = 356, year = 2007 }