%0 Journal Article %1 lu2023vicia %A Lu, J. %A Dreyer, I. %A Dickinson, M. S. %A Panzer, S. %A Jaslan, D. %A Navarro-Retamal, C. %A Geiger, D. %A Terpitz, U. %A Becker, D. %A Stroud, R. M. %A Marten, I. %A Hedrich, R. %D 2023 %J Elife %K Vacuoles myOwn uni_network %R 10.7554/eLife.86384 %T Vicia faba SV channel VfTPC1 is a hyperexcitable variant of plant vacuole Two Pore Channels %U https://www.ncbi.nlm.nih.gov/pubmed/37991833https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665017/pdf/elife-86384.pdf %V 12 %X To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca(2+). In our search for species-dependent functional TPC1 channel variants with different luminal Ca(2+) sensitivity, we found in total three acidic residues present in Ca(2+) sensor sites 2 and 3 of the Ca(2+)-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca(2+). When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca(2+) sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca(2+) sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.

@article{lu2023vicia, abstract = {To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca(2+). In our search for species-dependent functional TPC1 channel variants with different luminal Ca(2+) sensitivity, we found in total three acidic residues present in Ca(2+) sensor sites 2 and 3 of the Ca(2+)-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca(2+). When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca(2+) sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca(2+) sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.}, added-at = {2024-02-15T15:11:54.000+0100}, author = {Lu, J. and Dreyer, I. and Dickinson, M. S. and Panzer, S. and Jaslan, D. and Navarro-Retamal, C. and Geiger, D. and Terpitz, U. and Becker, D. and Stroud, R. M. and Marten, I. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/2854c0ae164d8c16afdee72a790de75aa/jvsi_all}, doi = {10.7554/eLife.86384}, interhash = {03d60e6c02ca0cffc6f1166c14b35d73}, intrahash = {854c0ae164d8c16afdee72a790de75aa}, issn = {2050-084X (Electronic) 2050-084X (Linking)}, journal = {Elife}, keywords = {Vacuoles myOwn uni_network}, note = {Lu, Jinping Dreyer, Ingo Dickinson, Miles Sasha Panzer, Sabine Jaslan, Dawid Navarro-Retamal, Carlos Geiger, Dietmar Terpitz, Ulrich Becker, Dirk Stroud, Robert M Marten, Irene Hedrich, Rainer eng Koselleck award HE 1640/42-1/Deutsche Forschungsgemeinschaft/ priority programs 'MAdLand - Molecular Adaptation to Land: Plant Evolution to Change' HE 1640/45-1/Deutsche Forschungsgemeinschaft/ priority programs 'MAdLand - Molecular Adaptation to Land: Plant Evolution to Change' BE1867/9-1/Deutsche Forschungsgemeinschaft/ doctoral fellowship/China Scholarship Council/ STIPET fellowship/Deutscher Akademischer Austauschdienst/ FONDEQUIP EQM160063/Comision Nacional de Investigacion Cientifica y Tecnologica/ ATE220043/Anilio-Anid/ 3170434/Fondo Nacional de Desarrollo Cientifico y Tecnologico/ 1220504/Fondo Nacional de Desarrollo Cientifico y Tecnologico/ England 2023/11/22 Elife. 2023 Nov 22;12:e86384. doi: 10.7554/eLife.86384.}, timestamp = {2024-02-15T15:11:54.000+0100}, title = {Vicia faba SV channel VfTPC1 is a hyperexcitable variant of plant vacuole Two Pore Channels}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/37991833https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10665017/pdf/elife-86384.pdf}, volume = 12, year = 2023 }