%0 Journal Article %1 Brun2009 %A Brun, Emilie %A Cloutier, Pierre %A Sicard-Roselli, Cécile %A Fromm, Michel %A Sanche, Léon %D 2009 %J J Phys Chem B %K chemistry;Electrons;Escherichiacoli Atmosphere;DNADamage;DNA chemistry;Vacuum;X-Rays Bacterial %N 29 %P 10008--10013 %R 10.1021/jp902540k %T Damage induced to DNA by low-energy (0-30 eV) electrons under vacuum and atmospheric conditions. %U http://dx.doi.org/10.1021/jp902540k %V 113 %X In this study, we show that it is possible to obtain data on DNA damage induced by low-energy (0-30 eV) electrons under atmospheric conditions. Five monolayer films of plasmid DNA (3197 base pairs) deposited on glass and gold substrates are irradiated with 1.5 keV X-rays in ultrahigh vacuum and under atmospheric conditions. The total damage is analyzed by agarose gel electrophoresis. The damage produced on the glass substrate is attributed to energy absorption from X-rays, whereas that produced on the gold substrate arises from energy absorption from both the X-ray beam and secondary electrons emitted from the gold surface. By analysis of the energy of these secondary electrons, 96\% are found to have energies below 30 eV with a distribution peaking at 1.4 eV. The differences in damage yields recorded with the gold and glass substrates is therefore essentially attributed to the interaction of low-energy electrons with DNA under vacuum and hydrated conditions. From these results, the G values for low-energy electrons are determined to be four and six strand breaks per 100 eV, respectively.

@article{Brun2009, abstract = {In this study, we show that it is possible to obtain data on DNA damage induced by low-energy (0-30 eV) electrons under atmospheric conditions. Five monolayer films of plasmid DNA (3197 base pairs) deposited on glass and gold substrates are irradiated with 1.5 keV X-rays in ultrahigh vacuum and under atmospheric conditions. The total damage is analyzed by agarose gel electrophoresis. The damage produced on the glass substrate is attributed to energy absorption from X-rays, whereas that produced on the gold substrate arises from energy absorption from both the X-ray beam and secondary electrons emitted from the gold surface. By analysis of the energy of these secondary electrons, 96\% are found to have energies below 30 eV with a distribution peaking at 1.4 eV. The differences in damage yields recorded with the gold and glass substrates is therefore essentially attributed to the interaction of low-energy electrons with DNA under vacuum and hydrated conditions. From these results, the G values for low-energy electrons are determined to be four and six strand breaks per 100 eV, respectively.}, added-at = {2011-07-21T21:36:18.000+0200}, author = {Brun, Emilie and Cloutier, Pierre and Sicard-Roselli, Cécile and Fromm, Michel and Sanche, Léon}, biburl = {https://www.bibsonomy.org/bibtex/260e444988b7ea6dd488a7f6b9c3216a5/crc_chus}, doi = {10.1021/jp902540k}, institution = {Laboratoire de Chimie Physique, CNRS UMR 8000, Université Paris-Sud 11, Bat. 350, 91405 Orsay Cedex, France.}, interhash = {0c94bbf923d3e482dd58514344018094}, intrahash = {60e444988b7ea6dd488a7f6b9c3216a5}, journal = {J Phys Chem B}, keywords = {chemistry;Electrons;Escherichiacoli Atmosphere;DNADamage;DNA chemistry;Vacuum;X-Rays Bacterial}, language = {eng}, medline-pst = {ppublish}, month = Jul, number = 29, pages = {10008--10013}, pmid = {19603845}, timestamp = {2011-07-21T21:36:18.000+0200}, title = {Damage induced to DNA by low-energy (0-30 eV) electrons under vacuum and atmospheric conditions.}, url = {http://dx.doi.org/10.1021/jp902540k}, volume = 113, year = 2009 }