%0 Journal Article %1 citeulike:671176 %A Kreuzer, M. P. %A O’sullivan, C. K. %A Pravda, M. %A Guilbault, G. G. %D 2001 %J Analytica Chimica Acta %K a-p immunoassay immunosensor electrochemistry elisa %N 1 %P 45--53 %R 10.1016/S0003-2670(01)01137-0 %T Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples %U http://www.sciencedirect.com/science/article/B6TF4-43RV9KY-5/2/6b28a768a1b945c85d37399e983c0d64 %V 442 %X The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml-1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.

@article{citeulike:671176, abstract = {The current work describes the optimisation of a system for measurement of allergy antibodies (IgE) in blood samples using disposable screen-printed carbon electrode technology and amperometric detection of p-aminophenol, produced by alkaline phosphatase, at +300 mV versus Ag/AgCl. The system was primarily developed by enzyme-linked immunosorbent assay (ELISA) prior to analysis by screen-printed electrodes. The sensor incorporates the clinically relevant range of IgE in sensitised patients with a detection limit of 0.09 ng ml-1 IgE. The system is simple, requiring only addition of analyte (sample) and analysis is complete in 30 min. In addition, it is cost effective and was developed for use as a point of care device, capable of measuring IgE levels in a drop of blood.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Kreuzer, M. P. and O\’sullivan, C. K. and Pravda, M. and Guilbault, G. G.}, biburl = {https://www.bibsonomy.org/bibtex/2778792ee2e274faea22e6321947b494b/biblio24}, citeulike-article-id = {671176}, doi = {10.1016/S0003-2670(01)01137-0}, interhash = {0c1800a6f619d5b6162be7a5a7531f99}, intrahash = {778792ee2e274faea22e6321947b494b}, journal = {Analytica Chimica Acta}, keywords = {a-p immunoassay immunosensor electrochemistry elisa}, month = {August}, number = 1, pages = {45--53}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Development of an immunosensor for the determination of allergy antibody (IgE) in blood samples}, url = {http://www.sciencedirect.com/science/article/B6TF4-43RV9KY-5/2/6b28a768a1b945c85d37399e983c0d64}, volume = 442, year = 2001 }