We describe a clinically useful ligand displacement immunoassay for the measurement of serum or plasma phenobarbital and phenytoin. A conjugate of drug-specific antiserum covalently bound to micro-crystalline cellulose and a beta-galactosidase-labelled drug derivative is prepared as a lyophilized reagent. Sample is added to the conjugate and, after 5-min incubation at room temperature, the bound and displaced enzyme-ligand are separated by brief centrifugation. The enzyme activity of the displaced enzyme-ligand is measured on a spectrophotometer or centrifugal analyser. Linear calibration curves are obtained with appropriate sample dilution, allowing generation of the standard curve with a saline zero reference and a single calibrator. The performance of the assay compared favourably with other immunoassays; comparison studies with clinical specimens gave a correlation with EMIT of 0.96 (n = 122) for phenobarbital and 0.95 (n = 96) for phenytoin.
%0 Journal Article
%1 citeulike:578280
%A Hinds, J. A.
%A Pincombe, C. F.
%A Smith, S.
%A Duffy, P.
%D 1985
%J Clinica Chimica Acta
%K displacement immunoassay
%N 2-3
%P 105--115
%R 10.1016/0009-8981(85)90324-9
%T Ligand displacement immunoassay--demonstration of its use for the measurement of serum phenobarbital and phenytoin.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3928203
%V 149
%X We describe a clinically useful ligand displacement immunoassay for the measurement of serum or plasma phenobarbital and phenytoin. A conjugate of drug-specific antiserum covalently bound to micro-crystalline cellulose and a beta-galactosidase-labelled drug derivative is prepared as a lyophilized reagent. Sample is added to the conjugate and, after 5-min incubation at room temperature, the bound and displaced enzyme-ligand are separated by brief centrifugation. The enzyme activity of the displaced enzyme-ligand is measured on a spectrophotometer or centrifugal analyser. Linear calibration curves are obtained with appropriate sample dilution, allowing generation of the standard curve with a saline zero reference and a single calibrator. The performance of the assay compared favourably with other immunoassays; comparison studies with clinical specimens gave a correlation with EMIT of 0.96 (n = 122) for phenobarbital and 0.95 (n = 96) for phenytoin.
@article{citeulike:578280,
abstract = {We describe a clinically useful ligand displacement immunoassay for the measurement of serum or plasma phenobarbital and phenytoin. A conjugate of drug-specific antiserum covalently bound to micro-crystalline cellulose and a beta-galactosidase-labelled drug derivative is prepared as a lyophilized reagent. Sample is added to the conjugate and, after 5-min incubation at room temperature, the bound and displaced enzyme-ligand are separated by brief centrifugation. The enzyme activity of the displaced enzyme-ligand is measured on a spectrophotometer or centrifugal analyser. Linear calibration curves are obtained with appropriate sample dilution, allowing generation of the standard curve with a saline zero reference and a single calibrator. The performance of the assay compared favourably with other immunoassays; comparison studies with clinical specimens gave a correlation with EMIT of 0.96 (n = 122) for phenobarbital and 0.95 (n = 96) for phenytoin.},
added-at = {2006-07-07T01:10:50.000+0200},
author = {Hinds, J. A. and Pincombe, C. F. and Smith, S. and Duffy, P.},
biburl = {https://www.bibsonomy.org/bibtex/2e5a4646c3e84d71b889b2d50377a540b/biblio24},
citeulike-article-id = {578280},
doi = {10.1016/0009-8981(85)90324-9},
interhash = {1b41d4b26235d84941765a1c56bf8811},
intrahash = {e5a4646c3e84d71b889b2d50377a540b},
issn = {0009-8981},
journal = {Clinica Chimica Acta},
keywords = {displacement immunoassay},
month = {July},
number = {2-3},
pages = {105--115},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Ligand displacement immunoassay--demonstration of its use for the measurement of serum phenobarbital and phenytoin.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3928203},
volume = 149,
year = 1985
}