%0 Journal Article %1 citeulike:505023 %A Walmsley, T. A. %A Grant, S. %A George, P. M. %C Biochemistry Department, Princess Margaret Hospital, Christchurch, New Zealand. %D 1991 %J Clin Chem %K surfactant immunoturbidimetry apob %N 5 %P 748--753 %T Effect of plasma triglyceride concentrations on the accuracy of immunoturbidimetric assays of apolipoprotein B. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2032330 %V 37 %X We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in these reagent systems, and can be eliminated by including Tween 20 (2 g/L) in the reagent buffer. We have developed, and describe, an automated immunoturbidimetric method that allows the accurate determination of apolipoprotein B in the plasma of patients with hypertriglyceridemia.

@article{citeulike:505023, abstract = {We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in these reagent systems, and can be eliminated by including Tween 20 (2 g/L) in the reagent buffer. We have developed, and describe, an automated immunoturbidimetric method that allows the accurate determination of apolipoprotein B in the plasma of patients with hypertriglyceridemia.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Biochemistry Department, Princess Margaret Hospital, Christchurch, New Zealand.}, author = {Walmsley, T. A. and Grant, S. and George, P. M.}, biburl = {https://www.bibsonomy.org/bibtex/2526af7dce47d21ae2a4ebf11ff18662c/biblio24}, citeulike-article-id = {505023}, interhash = {209dcf1db12977be26ea501684a83799}, intrahash = {526af7dce47d21ae2a4ebf11ff18662c}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {surfactant immunoturbidimetry apob}, month = May, number = 5, pages = {748--753}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Effect of plasma triglyceride concentrations on the accuracy of immunoturbidimetric assays of apolipoprotein B.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=2032330}, volume = 37, year = 1991 }