%0 Journal Article %1 citeulike:489674 %A Lovegrove, J. A. %A Isherwood, S. G. %A Jackson, K. G. %A Williams, C. M. %A Gould, B. J. %C Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford, UK. %D 1996 %J Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism %K immunoassay elisa apob %N 3 %P 221--229 %R 10.1016/0005-2760(96)00039-2 %T Quantitation of apolipoprotein B-48 in triacylglycerol-rich lipoproteins by a specific enzyme-linked immunosorbent assay. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8664332 %V 1301 %X This paper describes the use of an antiserum, specific for apolipoprotein (apo) B-48, in a competitive, enzyme-linked immunosorbent assay (ELISA) for apo B-48 in triacylglycerol-rich lipoprotein (TRL) fractions prepared from fasting and post-prandial plasma samples. Previously we showed the antiserum to act as an effective immunoblotting agent following sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Its use in this ELISA indicates that the antiserum recognises the C-terminal region of the protein on the surface of lipoprotein particles. The ELISA had a sensitivity of less than 37 ng/ml and intra- and inter-assay coefficients of variation of 3.8% and 8.6%, respectively. There was no cross-reaction in the ELISA against serum albumin, ovalbumin, thyroglobulin, or apo B-100 (purified by immunoaffinity chromatography), and high lipid concentrations (as Intralipid) did not interfere. A low density lipoprotein fraction reacted in the ELISA but SDS-PAGE-Western blot analysis confirmed the presence, in the fraction, of a small amount of apo B-48, indicating the existence of low density dietary-derived lipoprotein particles. ELISA and SDS-PAGE-Western blot analysis were used to measure apo B-48 in 12 series of postprandial samples collected from 4 diabetic and 8 normal subjects, following test meals of varying fat content. The mean correlation between the two methods was r = 0.74. The mean fasting concentration of apo B-48 in the TRL fractions from 15 healthy men was 0.46 microgram/ml of plasma.

@article{citeulike:489674, abstract = {This paper describes the use of an antiserum, specific for apolipoprotein (apo) B-48, in a competitive, enzyme-linked immunosorbent assay (ELISA) for apo B-48 in triacylglycerol-rich lipoprotein (TRL) fractions prepared from fasting and post-prandial plasma samples. Previously we showed the antiserum to act as an effective immunoblotting agent following sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Its use in this ELISA indicates that the antiserum recognises the C-terminal region of the protein on the surface of lipoprotein particles. The ELISA had a sensitivity of less than 37 ng/ml and intra- and inter-assay coefficients of variation of 3.8% and 8.6%, respectively. There was no cross-reaction in the ELISA against serum albumin, ovalbumin, thyroglobulin, or apo B-100 (purified by immunoaffinity chromatography), and high lipid concentrations (as Intralipid) did not interfere. A low density lipoprotein fraction reacted in the ELISA but SDS-PAGE-Western blot analysis confirmed the presence, in the fraction, of a small amount of apo B-48, indicating the existence of low density dietary-derived lipoprotein particles. ELISA and SDS-PAGE-Western blot analysis were used to measure apo B-48 in 12 series of postprandial samples collected from 4 diabetic and 8 normal subjects, following test meals of varying fat content. The mean correlation between the two methods was r = 0.74. The mean fasting concentration of apo B-48 in the TRL fractions from 15 healthy men was 0.46 microgram/ml of plasma.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Centre for Nutrition and Food Safety, School of Biological Sciences, University of Surrey, Guildford, UK.}, author = {Lovegrove, J. A. and Isherwood, S. G. and Jackson, K. G. and Williams, C. M. and Gould, B. J.}, biburl = {https://www.bibsonomy.org/bibtex/2a14307092be2a07d02399eebe06b73f4/biblio24}, citeulike-article-id = {489674}, doi = {10.1016/0005-2760(96)00039-2}, interhash = {1330f552661002d87e4ec8e232e051c1}, intrahash = {a14307092be2a07d02399eebe06b73f4}, issn = {0006-3002}, journal = {Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism}, keywords = {immunoassay elisa apob}, month = {June}, number = 3, pages = {221--229}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Quantitation of apolipoprotein B-48 in triacylglycerol-rich lipoproteins by a specific enzyme-linked immunosorbent assay.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8664332}, volume = 1301, year = 1996 }