%0 Journal Article %1 Barb254 %A Barb, C. Richard %A Hausman, Gary J. %A Rekaya, Romdhane %A Lents, Clay A. %A Lkhagvadorj, Sender %A Qu, L. %A Cai, W. %A Couture, Oliver P. %A Anderson, Lloyd L. %A Dekkers, Jack C. M. %A Tuggle, Christopher K. %D 2010 %I American Physiological Society %J Physiological Genomics %K 2010 genomics pigs %N 3 %P 254--268 %R 10.1152/physiolgenomics.00006.2010 %T Gene expression in hypothalamus, liver, and adipose tissues and food intake response to melanocortin-4 receptor agonist in pigs expressing melanocortin-4 receptor mutations %U http://physiolgenomics.physiology.org/content/41/3/254 %V 41 %X Transcriptional profiling was used to identify genes and pathways that responded to intracerebroventricular injection of melanocortin-4 receptor (MC4R) agonist Nle4, d-Phe7-α-melanocyte stimulating hormone (NDP-MSH) in pigs homozygous for the missense mutation in the MC4R, D298 allele (n = 12), N298 allele (n = 12), or heterozygous (n = 12). Food intake (FI) was measured at 12 and 24 h after treatment. All pigs were killed at 24 h after treatment, and hypothalamus, liver, and back-fat tissue was collected. NDP-MSH suppressed (P \< 0.004) FI at 12 and 24 h in all animals after treatment. In response to NDP-MSH, 278 genes in hypothalamus (q <= 0.07, P <= 0.001), 249 genes in liver (q <= 0.07, P <= 0.001), and 5,066 genes in fat (q <= 0.07, P <= 0.015) were differentially expressed. Pathway analysis of NDP-MSH-induced differentially expressed genes indicated that genes involved in cell communication, nucleotide metabolism, and signal transduction were prominently downregulated in the hypothalamus. In both liver and adipose tissue, energy-intensive biosynthetic and catabolic processes were downregulated in response to NDP-MSH. This included genes encoding for biosynthetic pathways such as steroid and lipid biosynthesis, fatty acid synthesis, and amino acid synthesis. Genes involved in direct energy-generating processes, such as oxidative phosphorylation, electron transport, and ATP synthesis, were upregulated, whereas TCA-associated genes were prominently downregulated in NDP-MSH-treated pigs. Our data also indicate a metabolic switch toward energy conservation since genes involved in energy-intensive biosynthetic and catabolic processes were downregulated in NDP-MSH-treated pigs.

@article{Barb254, abstract = {Transcriptional profiling was used to identify genes and pathways that responded to intracerebroventricular injection of melanocortin-4 receptor (MC4R) agonist [Nle4, d-Phe7]-α-melanocyte stimulating hormone (NDP-MSH) in pigs homozygous for the missense mutation in the MC4R, D298 allele (n = 12), N298 allele (n = 12), or heterozygous (n = 12). Food intake (FI) was measured at 12 and 24 h after treatment. All pigs were killed at 24 h after treatment, and hypothalamus, liver, and back-fat tissue was collected. NDP-MSH suppressed (P \< 0.004) FI at 12 and 24 h in all animals after treatment. In response to NDP-MSH, 278 genes in hypothalamus (q <= 0.07, P <= 0.001), 249 genes in liver (q <= 0.07, P <= 0.001), and 5,066 genes in fat (q <= 0.07, P <= 0.015) were differentially expressed. Pathway analysis of NDP-MSH-induced differentially expressed genes indicated that genes involved in cell communication, nucleotide metabolism, and signal transduction were prominently downregulated in the hypothalamus. In both liver and adipose tissue, energy-intensive biosynthetic and catabolic processes were downregulated in response to NDP-MSH. This included genes encoding for biosynthetic pathways such as steroid and lipid biosynthesis, fatty acid synthesis, and amino acid synthesis. Genes involved in direct energy-generating processes, such as oxidative phosphorylation, electron transport, and ATP synthesis, were upregulated, whereas TCA-associated genes were prominently downregulated in NDP-MSH-treated pigs. Our data also indicate a metabolic switch toward energy conservation since genes involved in energy-intensive biosynthetic and catabolic processes were downregulated in NDP-MSH-treated pigs.}, added-at = {2016-05-15T21:33:57.000+0200}, author = {Barb, C. Richard and Hausman, Gary J. and Rekaya, Romdhane and Lents, Clay A. and Lkhagvadorj, Sender and Qu, L. and Cai, W. and Couture, Oliver P. and Anderson, Lloyd L. and Dekkers, Jack C. M. and Tuggle, Christopher K.}, biburl = {https://www.bibsonomy.org/bibtex/2eeaa3d0fb57ffe171294b725b2fe6734/uga.abgg}, doi = {10.1152/physiolgenomics.00006.2010}, eprint = {http://physiolgenomics.physiology.org/content/41/3/254.full.pdf}, interhash = {139173f8df62f751b016246004b9ca32}, intrahash = {eeaa3d0fb57ffe171294b725b2fe6734}, issn = {1094-8341}, journal = {Physiological Genomics}, keywords = {2010 genomics pigs}, number = 3, pages = {254--268}, publisher = {American Physiological Society}, timestamp = {2016-05-15T21:33:57.000+0200}, title = {Gene expression in hypothalamus, liver, and adipose tissues and food intake response to melanocortin-4 receptor agonist in pigs expressing melanocortin-4 receptor mutations}, url = {http://physiolgenomics.physiology.org/content/41/3/254}, volume = 41, year = 2010 }