Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82\% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.
%0 Journal Article
%1 mellmann_sequence-based_2004
%A Mellmann, Alexander
%A Mosters, Jan
%A Bartelt, Edda
%A Roggentin, Peter
%A Ammon, Andrea
%A Friedrich, Alexander W
%A Karch, Helge
%A Harmsen, Dag
%D 2004
%J Journal of Clinical Microbiology
%K Analysis, Animals, Bacterial Campylobacter Campylobacter, Cattle, Disease Electrophoresis, Flagellin, Gel, Genotype, Humans, Infections, Outbreaks, Sequence Techniques, Typing {DNA} {Pulsed-Field,}
%N 10
%P 4840--4842
%R 10.1128/JCM.42.10.4840-4842.2004
%T Sequence-based typing of flaB is a more stable screening tool than typing of flaA for monitoring of Campylobacter populations
%U http://www.ncbi.nlm.nih.gov/pubmed/15472357
%V 42
%X Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82\% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.
@article{mellmann_sequence-based_2004,
abstract = {Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of {flaB),} and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82\% {(PFGE-flaA} or {PFGE-flaB)} concordance. {flaA} was overdiscriminatory in three cases, most probably due to intragenomic recombination.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Mellmann, Alexander and Mosters, Jan and Bartelt, Edda and Roggentin, Peter and Ammon, Andrea and Friedrich, Alexander W and Karch, Helge and Harmsen, Dag},
biburl = {https://www.bibsonomy.org/bibtex/22beb7c18170867735b75292f1f2fd3f7/jelias},
doi = {10.1128/JCM.42.10.4840-4842.2004},
interhash = {39abe181a4b5db66bc7e1571e15795fc},
intrahash = {2beb7c18170867735b75292f1f2fd3f7},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {Analysis, Animals, Bacterial Campylobacter Campylobacter, Cattle, Disease Electrophoresis, Flagellin, Gel, Genotype, Humans, Infections, Outbreaks, Sequence Techniques, Typing {DNA} {Pulsed-Field,}},
month = oct,
note = {{PMID:} 15472357},
number = 10,
pages = {4840--4842},
timestamp = {2011-03-11T10:06:13.000+0100},
title = {Sequence-based typing of {flaB} is a more stable screening tool than typing of {flaA} for monitoring of Campylobacter populations},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15472357},
volume = 42,
year = 2004
}