BACKGROUND: Circulating endothelial cells (CECs) have been identified as markers of vascular damage in a variety of disorders, such as myocardial infarction, vasculitis, and transplantation. CD146-driven immunomagnetic isolation has gained widespread use, but the technique is hampered by the lack of a definition of CECs and the absence of a consensus for their enumeration. AIM: To evaluate several variables influencing immunomagnetic isolation of CECs, formulate a definition for CECs and propose a consensus protocol for their enumeration. METHODS: We devised a protocol based on CD146-driven immunomagnetic isolation and a subsequent confirmatory step with Ulex-Europaeus-Lectin-1 staining. In a multi-center effort, we evaluated the preanalytical and analytical phases of this protocol. We evaluated the effects of storage, anticoagulation and density centrifugation, and compiled previous experience with this technique. RESULTS: Our protocol permitted unequivocal identification of CECs with
%0 Journal Article
%1 Woywodt.2006
%A Woywodt, A.
%A Blann, A. D.
%A Kirsch, T.
%A Erdbruegger, U.
%A Banzet, N.
%A Haubitz, M.
%A gnat George, F.
%D 2006
%J J.Thromb.Haemost.
%K Antigens CD146 Cell Cells Chemistry Count Diseases Endothelial Humans Immunomagnetic Immunophenotyping Lectins Plant Reproducibility Results Separation Size Vascular analysis cells immunology methods of pathology
%N 3
%P 671-677
%T Isolation and enumeration of circulating endothelial cells by immunomagnetic isolation: proposal of a definition and a consensus protocol
%U PM:16460450
%V 4
%X BACKGROUND: Circulating endothelial cells (CECs) have been identified as markers of vascular damage in a variety of disorders, such as myocardial infarction, vasculitis, and transplantation. CD146-driven immunomagnetic isolation has gained widespread use, but the technique is hampered by the lack of a definition of CECs and the absence of a consensus for their enumeration. AIM: To evaluate several variables influencing immunomagnetic isolation of CECs, formulate a definition for CECs and propose a consensus protocol for their enumeration. METHODS: We devised a protocol based on CD146-driven immunomagnetic isolation and a subsequent confirmatory step with Ulex-Europaeus-Lectin-1 staining. In a multi-center effort, we evaluated the preanalytical and analytical phases of this protocol. We evaluated the effects of storage, anticoagulation and density centrifugation, and compiled previous experience with this technique. RESULTS: Our protocol permitted unequivocal identification of CECs with
@article{Woywodt.2006,
abstract = {BACKGROUND: Circulating endothelial cells (CECs) have been identified as markers of vascular damage in a variety of disorders, such as myocardial infarction, vasculitis, and transplantation. CD146-driven immunomagnetic isolation has gained widespread use, but the technique is hampered by the lack of a definition of CECs and the absence of a consensus for their enumeration. AIM: To evaluate several variables influencing immunomagnetic isolation of CECs, formulate a definition for CECs and propose a consensus protocol for their enumeration. METHODS: We devised a protocol based on CD146-driven immunomagnetic isolation and a subsequent confirmatory step with Ulex-Europaeus-Lectin-1 staining. In a multi-center effort, we evaluated the preanalytical and analytical phases of this protocol. We evaluated the effects of storage, anticoagulation and density centrifugation, and compiled previous experience with this technique. RESULTS: Our protocol permitted unequivocal identification of CECs with},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Woywodt, A. and Blann, A. D. and Kirsch, T. and Erdbruegger, U. and Banzet, N. and Haubitz, M. and gnat George, F.},
biburl = {https://www.bibsonomy.org/bibtex/20f2c2b787263484387583f94f1ac9608/kanefendt},
interhash = {439a873d16556b9a3416bc5ac6109448},
intrahash = {0f2c2b787263484387583f94f1ac9608},
journal = {J.Thromb.Haemost.},
keywords = {Antigens CD146 Cell Cells Chemistry Count Diseases Endothelial Humans Immunomagnetic Immunophenotyping Lectins Plant Reproducibility Results Separation Size Vascular analysis cells immunology methods of pathology},
number = 3,
pages = {671-677},
timestamp = {2010-02-05T11:28:54.000+0100},
title = {Isolation and enumeration of circulating endothelial cells by immunomagnetic isolation: proposal of a definition and a consensus protocol},
url = {PM:16460450},
volume = 4,
year = 2006
}