%0 Journal Article %1 Schübeler04 %A Schübeler, Dirk %A MacAlpine, David M %A Scalzo, David %A Wirbelauer, Christiane %A Kooperberg, Charles %A van Leeuwen, Fred %A Gottschling, Daniel E %A O'Neill, Laura P %A Turner, Bryan M %A Delrow, Jeffrey %A Bell, Stephen P %A Groudine, Mark %D 2004 %J Genes Dev. %K Acetylation Animals Chromatin,_genetics/metabolism DNA_Replication Drosophila_Proteins,_genetics Drosophila_melanogaster,_genetics Eukaryotic_Cells,_physiology Gene_Expression_Profiling,_methods Genome Histones,_genetics/metabolism Methylation Oligonucleotide_Array_Sequence_Analysis RNA,_biosynthesis Transcription,_Genetic %N 11 %P 1263-71 %R 10.1101/gad.1198204 %T The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote. %U http://dx.doi.org/10.1101/gad.1198204 %V 18 %X The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity. To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome-wide chromatin structure analysis in a higher eukaryote. We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues. Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity.

@article{Schübeler04, abstract = {The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity. To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome-wide chromatin structure analysis in a higher eukaryote. We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues. Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity.}, added-at = {2010-01-26T20:35:53.000+0100}, author = {Sch{\"{u}}beler, Dirk and MacAlpine, David M and Scalzo, David and Wirbelauer, Christiane and Kooperberg, Charles and van Leeuwen, Fred and Gottschling, Daniel E and O'Neill, Laura P and Turner, Bryan M and Delrow, Jeffrey and Bell, Stephen P and Groudine, Mark}, biburl = {https://www.bibsonomy.org/bibtex/2d6247320b0f6347964459c96dbccbf78/denilw}, doi = {10.1101/gad.1198204}, file = {article:../Histone modifications/The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.pdf:pdf}, institution = {Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. dirk@fmi.ch}, interhash = {47711ca0c7704fb3e921c909790a36e9}, intrahash = {d6247320b0f6347964459c96dbccbf78}, journal = {Genes Dev.}, keywords = {Acetylation Animals Chromatin,_genetics/metabolism DNA_Replication Drosophila_Proteins,_genetics Drosophila_melanogaster,_genetics Eukaryotic_Cells,_physiology Gene_Expression_Profiling,_methods Genome Histones,_genetics/metabolism Methylation Oligonucleotide_Array_Sequence_Analysis RNA,_biosynthesis Transcription,_Genetic}, month = Jun, number = 11, owner = {denilw}, pages = {1263-71}, pii = {18/11/1263}, pmid = {15175259}, timestamp = {2010-01-26T20:36:03.000+0100}, title = {The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote.}, url = {http://dx.doi.org/10.1101/gad.1198204}, volume = 18, year = 2004 }