In the past, molecular mechanisms that drive the initiation of an inflammatory response have been studied intensively. However, corresponding mechanisms that sustain the expression of inflammatory response genes and hence contribute to the establishment of chronic disorders remain poorly understood. Recently, we provided genetic evidence that signaling via the receptor for advanced glycation end products (Rage) drives the strength and maintenance of an inflammatory reaction. In order to decipher the mode of Rage function on gene transcription levels during inflammation, we applied global gene expression profiling on time-resolved samples of mouse back skin, which had been treated with the phorbol ester TPA, a potent inducer of skin inflammation.Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels. Next, we used a data set of differentially expressed genes between TPA-treated wt and Rage-deficient skin and performed computational analysis of their proximal promoter regions. We found a highly significant enrichment for several transcription factor binding sites (TFBS) leading to the prediction that corresponding transcription factors, such as Sp1, Tcfap2, E2f, Myc and Egr, are regulated by Rage signaling. Accordingly, we could confirm aberrant expression and regulation of members of the E2f protein family in epidermal keratinocytes of Rage-deficient mice.In summary, our data support the model that engagement of Rage converts a transient cellular stimulation into sustained cellular dysfunction and highlight a novel role of the Rb-E2f pathway in Rage-dependent inflammation during pathological conditions.
%0 Journal Article
%1 Riehl2010
%A Riehl, Astrid
%A Bauer, Tobias
%A Brors, Benedikt
%A Busch, Hauke
%A Mark, Regina
%A Németh, Julia
%A Gebhardt, Christoffer
%A Bierhaus, Angelika
%A Nawroth, Peter
%A Eils, Roland
%A König, Rainer
%A Angel, Peter
%A Hess, Jochen
%D 2010
%J BMC Genomics
%K Acetate, Analysis; Animal; Animals; Biological; Biology; Chain Cluster Computational Disease E2F Expression Factors Factors, Gene Genetic, Immunologic, Inflammation, Mice; Models, Networks, Polymerase Profiling; Promoter Protein, Reaction; Receptors, Regions, Regulation, Regulatory Retinoblastoma Reverse Signal Skin, Tetradecanoylphorbol Time Transcriptase Transcription Transduction, deficiency/genetics/metabolism; drug effects; genetics/metabolism; genetics/pathology; genetics; metabolism/pathology; metabolism; pharmacology;
%P 537
%R 10.1186/1471-2164-11-537
%T Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation.
%U http://dx.doi.org/10.1186/1471-2164-11-537
%V 11
%X In the past, molecular mechanisms that drive the initiation of an inflammatory response have been studied intensively. However, corresponding mechanisms that sustain the expression of inflammatory response genes and hence contribute to the establishment of chronic disorders remain poorly understood. Recently, we provided genetic evidence that signaling via the receptor for advanced glycation end products (Rage) drives the strength and maintenance of an inflammatory reaction. In order to decipher the mode of Rage function on gene transcription levels during inflammation, we applied global gene expression profiling on time-resolved samples of mouse back skin, which had been treated with the phorbol ester TPA, a potent inducer of skin inflammation.Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels. Next, we used a data set of differentially expressed genes between TPA-treated wt and Rage-deficient skin and performed computational analysis of their proximal promoter regions. We found a highly significant enrichment for several transcription factor binding sites (TFBS) leading to the prediction that corresponding transcription factors, such as Sp1, Tcfap2, E2f, Myc and Egr, are regulated by Rage signaling. Accordingly, we could confirm aberrant expression and regulation of members of the E2f protein family in epidermal keratinocytes of Rage-deficient mice.In summary, our data support the model that engagement of Rage converts a transient cellular stimulation into sustained cellular dysfunction and highlight a novel role of the Rb-E2f pathway in Rage-dependent inflammation during pathological conditions.
@article{Riehl2010,
__markedentry = {[bbrors:6]},
abstract = {In the past, molecular mechanisms that drive the initiation of an inflammatory response have been studied intensively. However, corresponding mechanisms that sustain the expression of inflammatory response genes and hence contribute to the establishment of chronic disorders remain poorly understood. Recently, we provided genetic evidence that signaling via the receptor for advanced glycation end products (Rage) drives the strength and maintenance of an inflammatory reaction. In order to decipher the mode of Rage function on gene transcription levels during inflammation, we applied global gene expression profiling on time-resolved samples of mouse back skin, which had been treated with the phorbol ester TPA, a potent inducer of skin inflammation.Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels. Next, we used a data set of differentially expressed genes between TPA-treated wt and Rage-deficient skin and performed computational analysis of their proximal promoter regions. We found a highly significant enrichment for several transcription factor binding sites (TFBS) leading to the prediction that corresponding transcription factors, such as Sp1, Tcfap2, E2f, Myc and Egr, are regulated by Rage signaling. Accordingly, we could confirm aberrant expression and regulation of members of the E2f protein family in epidermal keratinocytes of Rage-deficient mice.In summary, our data support the model that engagement of Rage converts a transient cellular stimulation into sustained cellular dysfunction and highlight a novel role of the Rb-E2f pathway in Rage-dependent inflammation during pathological conditions.},
added-at = {2015-04-09T12:36:21.000+0200},
author = {Riehl, Astrid and Bauer, Tobias and Brors, Benedikt and Busch, Hauke and Mark, Regina and N{\'{e}}meth, Julia and Gebhardt, Christoffer and Bierhaus, Angelika and Nawroth, Peter and Eils, Roland and K{\"{o}}nig, Rainer and Angel, Peter and Hess, Jochen},
biburl = {https://www.bibsonomy.org/bibtex/2da69261d062ea3bb5d44746dc0716b25/bbrors},
doi = {10.1186/1471-2164-11-537},
institution = {Signal Transduction and Growth Control, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.},
interhash = {4a28ed95430f5887cd8d67336bc57fa6},
intrahash = {da69261d062ea3bb5d44746dc0716b25},
journal = {BMC Genomics},
keywords = {Acetate, Analysis; Animal; Animals; Biological; Biology; Chain Cluster Computational Disease E2F Expression Factors Factors, Gene Genetic, Immunologic, Inflammation, Mice; Models, Networks, Polymerase Profiling; Promoter Protein, Reaction; Receptors, Regions, Regulation, Regulatory Retinoblastoma Reverse Signal Skin, Tetradecanoylphorbol Time Transcriptase Transcription Transduction, deficiency/genetics/metabolism; drug effects; genetics/metabolism; genetics/pathology; genetics; metabolism/pathology; metabolism; pharmacology;},
language = {eng},
medline-pst = {epublish},
owner = {bbrors},
pages = 537,
pii = {1471-2164-11-537},
pmid = {20923549},
timestamp = {2015-04-09T12:36:21.000+0200},
title = {Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation.},
url = {http://dx.doi.org/10.1186/1471-2164-11-537},
volume = 11,
year = 2010
}