%0 Journal Article %1 citeulike:469349 %A Yu, B. S. %A Choi, Y. K. %A Chung, H. H. %D 1987 %J Biotechnol Appl Biochem %K l-i-l-a competition complement immunoassay homogeneous ag_detection liposome %N 3 %P 209--216 %T Development of immunoassay methods by use of liposomes. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3606827 %V 9 %X In order to develop liposomes for use in an immunoassay system, the preparation of immune liposomes and their characterization have been investigated. Liposomes have potential use in extremely sensitive analytical immunoassays, in addition to serving as an attractive drug delivery system. This liposome immunoassay system is based on membrane immunochemistry and an enzymatic reaction. An intense yellow color, easily detectable with the naked eye, was produced quite rapidly by the lysis of bovine serum albumin (BSA)-labeled, alkaline phosphatase-entrapped liposomes in the presence of anti-BSA rabbit serum and active complement under alkaline conditions. Sensitive detection is possible because of the antigen-antibody complex reaction, which leads to liposome lysis and an enzymatic reaction. The liposome immunoassay method offers a rapid, simple, and sensitive testing procedure which can quantitatively and qualitatively determine the presence or absence of antigenic materials and antibodies.

@article{citeulike:469349, abstract = {In order to develop liposomes for use in an immunoassay system, the preparation of immune liposomes and their characterization have been investigated. Liposomes have potential use in extremely sensitive analytical immunoassays, in addition to serving as an attractive drug delivery system. This liposome immunoassay system is based on membrane immunochemistry and an enzymatic reaction. An intense yellow color, easily detectable with the naked eye, was produced quite rapidly by the lysis of bovine serum albumin (BSA)-labeled, alkaline phosphatase-entrapped liposomes in the presence of anti-BSA rabbit serum and active complement under alkaline conditions. Sensitive detection is possible because of the antigen-antibody complex reaction, which leads to liposome lysis and an enzymatic reaction. The liposome immunoassay method offers a rapid, simple, and sensitive testing procedure which can quantitatively and qualitatively determine the presence or absence of antigenic materials and antibodies.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Yu, B. S. and Choi, Y. K. and Chung, H. H.}, biburl = {https://www.bibsonomy.org/bibtex/2f489662f3d43fe1f1e796e1c607ba9b2/biblio24}, citeulike-article-id = {469349}, interhash = {5349198688993fc1be426c5623f0b521}, intrahash = {f489662f3d43fe1f1e796e1c607ba9b2}, issn = {0885-4513}, journal = {Biotechnol Appl Biochem}, keywords = {l-i-l-a competition complement immunoassay homogeneous ag_detection liposome}, month = {June}, number = 3, pages = {209--216}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Development of immunoassay methods by use of liposomes.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3606827}, volume = 9, year = 1987 }