%0 Journal Article %1 pmid28489817 %A Flock, T. %A Hauser, A. S. %A Lund, N. %A Gloriam, D. E. %A Balaji, S. %A Babu, M. M. %D 2017 %J Nature %K imported %N 7654 %P 317--322 %T Selectivity determinants of GPCR-G-protein binding %V 545 %X The selective coupling of G-protein-coupled receptors (GPCRs) to specific G proteins is critical to trigger the appropriate physiological response. However, the determinants of selective binding have remained elusive. Here we reveal the existence of a selectivity barcode (that is, patterns of amino acids) on each of the 16 human G proteins that is recognized by distinct regions on the approximately 800 human receptors. Although universally conserved positions in the barcode allow the receptors to bind and activate G proteins in a similar manner, different receptors recognize the unique positions of the G-protein barcode through distinct residues, like multiple keys (receptors) opening the same lock (G protein) using non-identical cuts. Considering the evolutionary history of GPCRs allows the identification of these selectivity-determining residues. These findings lay the foundation for understanding the molecular basis of coupling selectivity within individual receptors and G proteins.

@article{pmid28489817, abstract = {The selective coupling of G-protein-coupled receptors (GPCRs) to specific G proteins is critical to trigger the appropriate physiological response. However, the determinants of selective binding have remained elusive. Here we reveal the existence of a selectivity barcode (that is, patterns of amino acids) on each of the 16 human G proteins that is recognized by distinct regions on the approximately 800 human receptors. Although universally conserved positions in the barcode allow the receptors to bind and activate G proteins in a similar manner, different receptors recognize the unique positions of the G-protein barcode through distinct residues, like multiple keys (receptors) opening the same lock (G protein) using non-identical cuts. Considering the evolutionary history of GPCRs allows the identification of these selectivity-determining residues. These findings lay the foundation for understanding the molecular basis of coupling selectivity within individual receptors and G proteins.}, added-at = {2021-01-14T21:21:20.000+0100}, author = {Flock, T. and Hauser, A. S. and Lund, N. and Gloriam, D. E. and Balaji, S. and Babu, M. M.}, biburl = {https://www.bibsonomy.org/bibtex/2eaad6505d97d5176a53cf86de9032dfd/mbgroup}, interhash = {67519a13e1856e7c256869b6a43101cf}, intrahash = {eaad6505d97d5176a53cf86de9032dfd}, journal = {Nature}, keywords = {imported}, month = {05}, number = 7654, pages = {317--322}, timestamp = {2021-01-14T21:21:20.000+0100}, title = {{{S}electivity determinants of {G}{P}{C}{R}-{G}-protein binding}}, volume = 545, year = 2017 }