%0 Journal Article %1 RN188 %A Del-Cid, A. %A Ubilla, P. %A Ravanal, M.C. %A Medina, E. %A Vaca, I. %A Levican, G. %A Eyzaguirre, J. %A Chavez, R. %D 2014 %J Applied Biochemistry and Biotechnology %K antarctic cladosporium cold-active dqcauchile fungi, marine sp., sponges, stability thermostability, xylanases, %N 1 %P 524-532 %R 10.1007/s12010-013-0551-1 %T Cold-Active Xylanase Produced by Fungi Associated with Antarctic Marine Sponges %U /brokenurl#<Go to ISI>://WOS:000331005200043 %V 172 %X Despite their potential biotechnological applications, cold-active xylanolytic enzymes have been poorly studied. In this work, 38 fungi isolated from marine sponges collected in King George Island, Antarctica, were screened as new sources of cold-active xylanases. All of them showed xylanase activity at 15 and 23 A degrees C in semiquantitative plate assays. One of these isolates, Cladosporium sp., showed the highest activity and was characterized in detail. Cladosporium sp. showed higher xylanolytic activity when grown on beechwood or birchwood xylan and wheat bran, but wheat straw and oat bran were not so good inducers of this activity. The optimal pH for xylanase activity was 6.0, although pH stability was slightly wider (pH 5-7). On the other hand, Cladosporium sp. showed high xylanase activity at low temperatures and very low thermal stability. Interestingly, thermal stability was even lower after culture media were removed and replaced by buffer, suggesting that low molecular component(s) of the culture media could be important in the stabilization of cold-active xylanase activity. To the best of our knowledge, this study is the first report on extracellular xylanase production by fungi associated with Antarctic marine sponges.

@article{RN188, abstract = {Despite their potential biotechnological applications, cold-active xylanolytic enzymes have been poorly studied. In this work, 38 fungi isolated from marine sponges collected in King George Island, Antarctica, were screened as new sources of cold-active xylanases. All of them showed xylanase activity at 15 and 23 A degrees C in semiquantitative plate assays. One of these isolates, Cladosporium sp., showed the highest activity and was characterized in detail. Cladosporium sp. showed higher xylanolytic activity when grown on beechwood or birchwood xylan and wheat bran, but wheat straw and oat bran were not so good inducers of this activity. The optimal pH for xylanase activity was 6.0, although pH stability was slightly wider (pH 5-7). On the other hand, Cladosporium sp. showed high xylanase activity at low temperatures and very low thermal stability. Interestingly, thermal stability was even lower after culture media were removed and replaced by buffer, suggesting that low molecular component(s) of the culture media could be important in the stabilization of cold-active xylanase activity. To the best of our knowledge, this study is the first report on extracellular xylanase production by fungi associated with Antarctic marine sponges.}, added-at = {2019-12-04T03:57:35.000+0100}, author = {Del-Cid, A. and Ubilla, P. and Ravanal, M.C. and Medina, E. and Vaca, I. and Levican, G. and Eyzaguirre, J. and Chavez, R.}, biburl = {https://www.bibsonomy.org/bibtex/2bafd9d5e388e34e51dd31c224875d1f6/dqcauchile}, doi = {10.1007/s12010-013-0551-1}, interhash = {6c3c6383277a2007c4bb0788893463e6}, intrahash = {bafd9d5e388e34e51dd31c224875d1f6}, issn = {0273-2289}, journal = {Applied Biochemistry and Biotechnology}, keywords = {antarctic cladosporium cold-active dqcauchile fungi, marine sp., sponges, stability thermostability, xylanases,}, number = 1, pages = {524-532}, timestamp = {2019-12-04T03:58:17.000+0100}, title = {Cold-Active Xylanase Produced by Fungi Associated with Antarctic Marine Sponges}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000331005200043}, volume = 172, year = 2014 }