%0 Journal Article %1 SUKHORUKOV1993 %A SUKHORUKOV, VL %A ARNOLD, WM %A ZIMMERMANN, U %C 175 FIFTH AVE, NEW YORK, NY 10010 %D 1993 %I SPRINGER VERLAG %J JOURNAL OF MEMBRANE BIOLOGY %K vladimir %N 1 %P 27-40 %T HYPOTONICALLY INDUCED CHANGES IN THE PLASMA-MEMBRANE OF CULTURED-MAMMALIAN-CELLS %V 132 %X Cells from three cell lines were electrorotated in media of osmotic strengths from 330 mOsm to 60 mOsm. From the field-frequency dependence of the rotation speed, the passive electrical properties of the surfaces were deduced. In all cases, the area-specific membrane capacitance (C(m)) decreased with osmolality. At 280 mOsm (iso-osmotic), SP2 (mouse myeloma) and G8 (hybridoma) cells had C(m) values of 1.01 +/- 0.04 muF/cm2 and 1.09 +/- 0.03 muF/cm2, respectively, whereas dispase-treated L-cells (sarcoma fibroblasts) exhibited C(m) = 2.18 +/- 0.10 muF/cm2. As the osmolality was reduced, the C(m) reached a well-defined minimum at 150 mOsm (SP2) or 180 mOsm (G8). Further reduction in osmolality gave a 7\% increase in C(m), after which a plateau close to 0.80 muF/cm2 was reached. However, the whole-cell capacities increased about twofold from 200 mOsm to 60 mOsm. L-cells showed very little change in C(m) between 280 mOsm and 150 mOsm, but below 150 mOsm the C(m) decreased rapidly. The changes in C(m) correlate well with the swelling of the cells assessed by means of van't Hoff plots. The apparent membrane conductance (including the effect of surface conductance) decreased with C(m), but then increased again instead of exhibiting a plateau. The rotation speed of the cells increased as the osmolality was lowered, and eventually attained almost the theoretical value. All measurements indicate that hypo-osmotically stressed cells obtain the necessary membrane area by using material from microvilli. However, below about 200 mOsm the whole-cell capacities indicate the progressive incorporation of `'extra'' membrane into the cell surface.

@article{SUKHORUKOV1993, abstract = {Cells from three cell lines were electrorotated in media of osmotic strengths from 330 mOsm to 60 mOsm. From the field-frequency dependence of the rotation speed, the passive electrical properties of the surfaces were deduced. In all cases, the area-specific membrane capacitance (C(m)) decreased with osmolality. At 280 mOsm (iso-osmotic), SP2 (mouse myeloma) and G8 (hybridoma) cells had C(m) values of 1.01 +/- 0.04 muF/cm2 and 1.09 +/- 0.03 muF/cm2, respectively, whereas dispase-treated L-cells (sarcoma fibroblasts) exhibited C(m) = 2.18 +/- 0.10 muF/cm2. As the osmolality was reduced, the C(m) reached a well-defined minimum at 150 mOsm (SP2) or 180 mOsm (G8). Further reduction in osmolality gave a 7\% increase in C(m), after which a plateau close to 0.80 muF/cm2 was reached. However, the whole-cell capacities increased about twofold from 200 mOsm to 60 mOsm. L-cells showed very little change in C(m) between 280 mOsm and 150 mOsm, but below 150 mOsm the C(m) decreased rapidly. The changes in C(m) correlate well with the swelling of the cells assessed by means of van't Hoff plots. The apparent membrane conductance (including the effect of surface conductance) decreased with C(m), but then increased again instead of exhibiting a plateau. The rotation speed of the cells increased as the osmolality was lowered, and eventually attained almost the theoretical value. All measurements indicate that hypo-osmotically stressed cells obtain the necessary membrane area by using material from microvilli. However, below about 200 mOsm the whole-cell capacities indicate the progressive incorporation of `'extra'' membrane into the cell surface.}, added-at = {2011-03-02T09:35:06.000+0100}, address = {175 FIFTH AVE, NEW YORK, NY 10010}, affiliation = {SUKHORUKOV, VL (Reprint Author), UNIV WURZBURG,BIOZENTRUM,DEPT BIOTECHNOL,AM HUBLAND,W-8700 WURZBURG,GERMANY.}, author = {SUKHORUKOV, VL and ARNOLD, WM and ZIMMERMANN, U}, biburl = {https://www.bibsonomy.org/bibtex/2ea7a4501297cc983465ce283f2be33fa/reichert}, doc-delivery-number = {KP931}, groups = {public}, interhash = {6eb5f94ea49a39728bf17c9617d3ac8d}, intrahash = {ea7a4501297cc983465ce283f2be33fa}, issn = {0022-2631}, journal = {JOURNAL OF MEMBRANE BIOLOGY}, journal-iso = {J. Membr. Biol.}, keywords = {vladimir}, keywords-plus = {DIELECTRICALLY OBSERVABLE CONSEQUENCES; ROTATING ELECTRIC-FIELD; DIFFUSIONAL MOTIONS; SINGLE CELLS; ELECTROFUSION; PROTOPLASTS; SURFACE; ERYTHROCYTES; HYBRIDOMAS; PARTICLES}, language = {English}, month = FEB, number = 1, number-of-cited-references = {41}, pages = {27-40}, publisher = {SPRINGER VERLAG}, subject-category = {Biochemistry \& Molecular Biology; Cell Biology; Physiology}, times-cited = {60}, timestamp = {2011-03-09T14:16:28.000+0100}, title = {HYPOTONICALLY INDUCED CHANGES IN THE PLASMA-MEMBRANE OF CULTURED-MAMMALIAN-CELLS}, type = {Article}, unique-id = {ISI:A1993KP93100003}, username = {reichert}, volume = 132, year = 1993 }