%0 Journal Article %1 Dudley2005Global %A Dudley, Aimee M. %A Janse, Daniel M. %A Tanay, Amos %A Shamir, Ron %A Church, George M. %D 2005 %I Nature Publishing Group %J Molecular Systems Biology %K gene-function phenotypes yeast-gene-dup %N 1 %P msb4100004-E1--msb4100004-E11 %R 10.1038/msb4100004 %T A global view of pleiotropy and phenotypically derived gene function in yeast %U http://dx.doi.org/10.1038/msb4100004 %V 1 %X Pleiotropy, the ability of a single mutant gene to cause multiple mutant phenotypes, is a relatively common but poorly understood phenomenon in biology. Perhaps the greatest challenge in the analysis of pleiotropic genes is determining whether phenotypes associated with a mutation result from the loss of a single function or of multiple functions encoded by the same gene. Here we estimate the degree of pleiotropy in yeast by measuring the phenotypes of 4710 mutants under 21 environmental conditions, finding that it is significantly higher than predicted by chance. We use a biclustering algorithm to group pleiotropic genes by common phenotype profiles. Comparisons of these clusters to biological process classifications, synthetic lethal interactions, and protein complex data support the hypothesis that this method can be used to genetically define cellular functions. Applying these functional classifications to pleiotropic genes, we are able to dissect phenotypes into groups associated with specific gene functions.

@article{Dudley2005Global, abstract = {Pleiotropy, the ability of a single mutant gene to cause multiple mutant phenotypes, is a relatively common but poorly understood phenomenon in biology. Perhaps the greatest challenge in the analysis of pleiotropic genes is determining whether phenotypes associated with a mutation result from the loss of a single function or of multiple functions encoded by the same gene. Here we estimate the degree of pleiotropy in yeast by measuring the phenotypes of 4710 mutants under 21 environmental conditions, finding that it is significantly higher than predicted by chance. We use a biclustering algorithm to group pleiotropic genes by common phenotype profiles. Comparisons of these clusters to biological process classifications, synthetic lethal interactions, and protein complex data support the hypothesis that this method can be used to genetically define cellular functions. Applying these functional classifications to pleiotropic genes, we are able to dissect phenotypes into groups associated with specific gene functions.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Dudley, Aimee M. and Janse, Daniel M. and Tanay, Amos and Shamir, Ron and Church, George M.}, biburl = {https://www.bibsonomy.org/bibtex/2020b6723570b49be1c9c1bec47d7e00a/karthikraman}, citeulike-article-id = {211040}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/msb4100004}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/msb4100004}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16729036}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16729036}, day = 29, doi = {10.1038/msb4100004}, interhash = {6faa6e6a56c8cbd660a5b943c6bb18c5}, intrahash = {020b6723570b49be1c9c1bec47d7e00a}, issn = {1744-4292}, journal = {Molecular Systems Biology}, keywords = {gene-function phenotypes yeast-gene-dup}, month = mar, number = 1, pages = {msb4100004-E1--msb4100004-E11}, pmid = {16729036}, posted-at = {2010-08-16 12:21:11}, priority = {2}, publisher = {Nature Publishing Group}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {A global view of pleiotropy and phenotypically derived gene function in yeast}, url = {http://dx.doi.org/10.1038/msb4100004}, volume = 1, year = 2005 }