%0 Journal Article %1 citeulike:480372 %A Meusel, M. %A Renneberg, R. %A Spener, F. %A Schmitz, G. %C Institute of Chemical and Biochemical Sensor Research, Münster, Germany. %D 1995 %J Biosens Bioelectron %K amperometry apoe sandwich immunoassay immunosensor ag_detection electrochemistry heterogeneous immunoelectrode %N 6-7 %P 577--586 %T Development of a heterogeneous amperometric immunosensor for the determination of apolipoprotein E in serum. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7612209 %V 10 %X Apolipoprotein (apo) E is a constituent of serum lipoproteins and can serve as a diagnostic parameter for the assessment of disorders of lipid metabolism. For the determination of apo E in serum a sandwich-type amperometric immunosensor using disposable membranes was developed. The best results were obtained by using a site-directed attachment of a monoclonal capture antibody to a hydrazide-functionalized membrane surface. Bound antigen was then determined with the aid of a polyclonal antibody labelled with alkaline phosphatase in conjunction with p-aminophenyl phosphate as substrate. In this approach a carbon working electrode (vs. Hg/HgCl2) was used, and enzymatically generated p-aminophenol could be monitored with a detection limit of 40 pmol and a linear range of 26-20000 nM. The sensor displayed a linear response from 50 to 1000 ng ml-1 apo E. In contrast, antibody coupling through primary amino groups led to a total loss of antigen binding capacity in this assay configuration. The approach with site-directed immobilization however, not only allowed the determination of apo E in serum, but also the determination of antibody cross-reactivity against the apolipoproteins AI, AII and B.

@article{citeulike:480372, abstract = {Apolipoprotein (apo) E is a constituent of serum lipoproteins and can serve as a diagnostic parameter for the assessment of disorders of lipid metabolism. For the determination of apo E in serum a sandwich-type amperometric immunosensor using disposable membranes was developed. The best results were obtained by using a site-directed attachment of a monoclonal capture antibody to a hydrazide-functionalized membrane surface. Bound antigen was then determined with the aid of a polyclonal antibody labelled with alkaline phosphatase in conjunction with p-aminophenyl phosphate as substrate. In this approach a carbon working electrode (vs. Hg/HgCl2) was used, and enzymatically generated p-aminophenol could be monitored with a detection limit of 40 pmol and a linear range of 26-20000 nM. The sensor displayed a linear response from 50 to 1000 ng ml-1 apo E. In contrast, antibody coupling through primary amino groups led to a total loss of antigen binding capacity in this assay configuration. The approach with site-directed immobilization however, not only allowed the determination of apo E in serum, but also the determination of antibody cross-reactivity against the apolipoproteins AI, AII and B.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Institute of Chemical and Biochemical Sensor Research, Münster, Germany.}, author = {Meusel, M. and Renneberg, R. and Spener, F. and Schmitz, G.}, biburl = {https://www.bibsonomy.org/bibtex/24450139fce1f4916c3b31e873da08353/biblio24}, citeulike-article-id = {480372}, interhash = {743d836304cc81c9de30dd7395232766}, intrahash = {4450139fce1f4916c3b31e873da08353}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {amperometry apoe sandwich immunoassay immunosensor ag_detection electrochemistry heterogeneous immunoelectrode}, number = {6-7}, pages = {577--586}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Development of a heterogeneous amperometric immunosensor for the determination of apolipoprotein E in serum.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=7612209}, volume = 10, year = 1995 }