%0 Journal Article %1 Magtanong2011Dosage %A Magtanong, Leslie %A Ho, Cheuk Hei H. %A Barker, Sarah L. %A Jiao, Wei %A Baryshnikova, Anastasia %A Bahr, Sondra %A Smith, Andrew M. %A Heisler, Lawrence E. %A Choy, John S. %A Kuzmin, Elena %A Andrusiak, Kerry %A Kobylianski, Anna %A Li, Zhijian %A Costanzo, Michael %A Basrai, Munira A. %A Giaever, Guri %A Nislow, Corey %A Andrews, Brenda %A Boone, Charles %D 2011 %I Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. %J Nature biotechnology %K interaction-networks yeast %N 6 %P 505--511 %R 10.1038/nbt.1855 %T Dosage suppression genetic interaction networks enhance functional wiring diagrams of the cell. %U http://dx.doi.org/10.1038/nbt.1855 %V 29 %X Dosage suppression is a genetic interaction in which overproduction of one gene rescues a mutant phenotype of another gene. Although dosage suppression is known to map functional connections among genes, the extent to which it might illuminate global cellular functions is unclear. Here we analyze a network of interactions linking dosage suppressors to 437 essential genes in yeast. For 424 genes, we curated interactions from the literature. Analyses revealed that many dosage suppression interactions occur between functionally related genes and that the majority do not overlap with other types of genetic or physical interactions. To confirm the generality of these network properties, we experimentally identified dosage suppressors for 29 genes from pooled populations of temperature-sensitive mutant cells transformed with a high-copy molecular-barcoded open reading frame library, MoBY-ORF 2.0. We classified 87\% of the 1,640 total interactions into four general types of suppression mechanisms, which provided insight into their relative frequencies. This work suggests that integrating the results of dosage suppression studies with other interaction networks could generate insights into the functional wiring diagram of a cell.

@article{Magtanong2011Dosage, abstract = { Dosage suppression is a genetic interaction in which overproduction of one gene rescues a mutant phenotype of another gene. Although dosage suppression is known to map functional connections among genes, the extent to which it might illuminate global cellular functions is unclear. Here we analyze a network of interactions linking dosage suppressors to 437 essential genes in yeast. For 424 genes, we curated interactions from the literature. Analyses revealed that many dosage suppression interactions occur between functionally related genes and that the majority do not overlap with other types of genetic or physical interactions. To confirm the generality of these network properties, we experimentally identified dosage suppressors for 29 genes from pooled populations of temperature-sensitive mutant cells transformed with a high-copy molecular-barcoded open reading frame library, {MoBY}-{ORF} 2.0. We classified 87\% of the 1,640 total interactions into four general types of suppression mechanisms, which provided insight into their relative frequencies. This work suggests that integrating the results of dosage suppression studies with other interaction networks could generate insights into the functional wiring diagram of a cell. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Magtanong, Leslie and Ho, Cheuk Hei H. and Barker, Sarah L. and Jiao, Wei and Baryshnikova, Anastasia and Bahr, Sondra and Smith, Andrew M. and Heisler, Lawrence E. and Choy, John S. and Kuzmin, Elena and Andrusiak, Kerry and Kobylianski, Anna and Li, Zhijian and Costanzo, Michael and Basrai, Munira A. and Giaever, Guri and Nislow, Corey and Andrews, Brenda and Boone, Charles}, biburl = {https://www.bibsonomy.org/bibtex/2fc5edaf239d215dd6a73b1874b1fe92e/karthikraman}, citeulike-article-id = {9307899}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.1855}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.1855}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/21572441}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=21572441}, day = 15, doi = {10.1038/nbt.1855}, interhash = {787fcc44417c4faa9597ee866afe5345}, intrahash = {fc5edaf239d215dd6a73b1874b1fe92e}, issn = {1546-1696}, journal = {Nature biotechnology}, keywords = {interaction-networks yeast}, month = jun, number = 6, pages = {505--511}, pmid = {21572441}, posted-at = {2011-06-01 12:34:21}, priority = {2}, publisher = {Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Dosage suppression genetic interaction networks enhance functional wiring diagrams of the cell.}, url = {http://dx.doi.org/10.1038/nbt.1855}, volume = 29, year = 2011 }