%0 Journal Article %1 hines2018developmental %A Hines, Rochelle M. %A Maric, Hans Michael %A Hines, Dustin J. %A Modgil, Amit %A Panzanelli, Patrizia %A Nakamura, Yasuko %A Nathanson, Anna J. %A Cross, Alan %A Deeb, Tarek %A Brandon, Nicholas J. %A Davies, Paul %A Fritschy, Jean-Marc %A Schindelin, Hermann %A Moss, Stephen J. %D 2018 %J Nature Communications %K maric %N 1 %P 3130-- %R 10.1038/s41467-018-05481-1 %T Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin %U https://doi.org/10.1038/s41467-018-05481-1 %V 9 %X Fast inhibitory synaptic transmission is mediated by γ-aminobutyric acid type A receptors (GABAARs) that are enriched at functionally diverse synapses via mechanisms that remain unclear. Using isothermal titration calorimetry and complementary methods we demonstrate an exclusive low micromolar binding of collybistin to the α2-subunit of GABAARs. To explore the biological relevance of collybistin-α2-subunit selectivity, we generate mice with a mutation in the α2-subunit-collybistin binding region (Gabra2-1). The mutation results in loss of a distinct subset of inhibitory synapses and decreased amplitude of inhibitory synaptic currents. Gabra2–1 mice have a striking phenotype characterized by increased susceptibility to seizures and early mortality. Surviving Gabra2-1 mice show anxiety and elevations in electroencephalogram δ power, which are ameliorated by treatment with the α2/α3-selective positive modulator, AZD7325. Taken together, our results demonstrate an α2-subunit selective binding of collybistin, which plays a key role in patterned brain activity, particularly during development.

@article{hines2018developmental, abstract = {Fast inhibitory synaptic transmission is mediated by γ-aminobutyric acid type A receptors (GABAARs) that are enriched at functionally diverse synapses via mechanisms that remain unclear. Using isothermal titration calorimetry and complementary methods we demonstrate an exclusive low micromolar binding of collybistin to the α2-subunit of GABAARs. To explore the biological relevance of collybistin-α2-subunit selectivity, we generate mice with a mutation in the α2-subunit-collybistin binding region (Gabra2-1). The mutation results in loss of a distinct subset of inhibitory synapses and decreased amplitude of inhibitory synaptic currents. Gabra2–1 mice have a striking phenotype characterized by increased susceptibility to seizures and early mortality. Surviving Gabra2-1 mice show anxiety and elevations in electroencephalogram δ power, which are ameliorated by treatment with the α2/α3-selective positive modulator, AZD7325. Taken together, our results demonstrate an α2-subunit selective binding of collybistin, which plays a key role in patterned brain activity, particularly during development.}, added-at = {2019-07-25T09:42:28.000+0200}, author = {Hines, Rochelle M. and Maric, Hans Michael and Hines, Dustin J. and Modgil, Amit and Panzanelli, Patrizia and Nakamura, Yasuko and Nathanson, Anna J. and Cross, Alan and Deeb, Tarek and Brandon, Nicholas J. and Davies, Paul and Fritschy, Jean-Marc and Schindelin, Hermann and Moss, Stephen J.}, biburl = {https://www.bibsonomy.org/bibtex/2ec5913407260a66cd528b2826f52748c/reichert}, doi = {10.1038/s41467-018-05481-1}, interhash = {8bfa0ee458a02759af08dac334059dd6}, intrahash = {ec5913407260a66cd528b2826f52748c}, issn = {20411723}, journal = {Nature Communications}, keywords = {maric}, number = 1, pages = {3130--}, refid = {Hines2018}, timestamp = {2019-07-25T09:42:28.000+0200}, title = {Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin}, url = {https://doi.org/10.1038/s41467-018-05481-1}, volume = 9, year = 2018 }